The ponderomotive force, exerted upon all dielectric liquids by a non-uniform electric field, can be used for the remote, voltage-controlled manipulation of 10 to 100μL volumes of cryogenic liquids. This liquid dielectrophoretic (DEP) effect, imposed by specially designed electrodes, combines with capillarity to influence the hydrostatic equilibria of liquid deuterium. A simple, one-dimensional...

ABSTRACT We present an experimental and numerical study of Joule heating in a polymeric insulator-based dielectrophoresis (iDEP) device. In this study, we used a microscale thermometry technique based upon the temperature-sensitive fluorescence of Rhodamine B dye to monitor the fluid temperature within our iDEP microfluidic devices. We then complemented our experiments with 3D finite element si...

Embryoid body (EB) formation forms an important step in embryonic stem cell differentiation invivo. In murine embryonic stem cell (mESC) cultures EB formation is inhibited by the inclusion of leukaemic inhibitory factor (LIF) in the medium. Assembly of mESCs into aggregates by positive dielectrophoresis (DEP) in high field regions between interdigitated oppositely castellated electrodes was fou...

Fig. S1 Layout of the D-DLD with peripheral vacuum pump and electronic connections. (a) The device is fabricated in PDMS and glass using replica molding. (b) Micrographs of the entrance channels and a side-wall. (c) A scale drawing of the separation device. A stream of particles injected into the device is continuously separated about the critical diameter, D c. Particles smaller than the criti...

The movement and behaviour of particles suspended in aqueous solutions subjected to non-uniform ac electric fields is examined. The ac electric fields induce movement of polarizable particles, a phenomenon known as dielectrophoresis. The high strength electric fields that are often used in separation systems can give rise to fluid motion, which in turn results in a viscous drag on the particle....

The contact phase of an assembly task involving micro and nano objects is complicated by the presence of surface and intermolecular forces such as electrostatic, surface-tension and Van der Waals forces. Assembly strategies must account for the presence of these forces in order to guarantee successful repeatable micro and nanoassemblies with high precision. A detailed model for this electrostat...

This paper presents a microfluidic pumping approach using traveling-wave dielectrophoresis (twDEP) of microparticles. With this approach, the flow is generated directly in the microfluidic devices by inducing strong electromechanical effects in the fluid using integrated microelectrodes. The fluidic driving mechanisms due to the particle-fluid and particle-particle interactions under twDEP are ...

Dielectrophoresis (DEP) has emerged as an important tool for the manipulation of bioparticles ranging from the submicron to the tens of microns in size. Here we show the use of phospholipid vesicle electroformation techniques to develop a new class of test particles with specifically engineered electrical propserties to enable identifiable dielectrophoretic responses in microfabricated systems....

Submicron particles such as latex spheres and viruses can be manipulated and characterized using dielectrophoresis. By the use of appropriate microelectrode arrays, particles can be trapped or moved between regions of high or low electric fields. The magnitude and direction of the dielectrophoretic force on the particle depends on its dielectric properties, so that a heterogeneous mixture of pa...

We present a novel technique to accurately position single cells on a substrate using negative dielectrophoresis and cell-substrate adhesion. The cells are suspended in physiological media throughout the patterning process. We also verify the biocompatibility of this method by demonstrating that the patterned cells proliferate and show normal morphology. We calculate the temperatures and transm...