The activity of a new, terpene-based formulation, code-named NT-VRL-1, against Human Coronavirus (HCoV) strain 229E was evaluated in human lung fibroblasts (MRC-5 cells), with and without the addition cannabidiol (CBD). main constituents terpene formulation used for experiment were beta caryophyllene, eucalyptol, citral. tested exhibited an antiviral effect when it pre-incubated host cells prio...

Enterohemorrhagic Escherichia coli (EHEC), such as E. coli O157:H7, are emerging food-borne pathogens worldwide. This micro-organism can damage the epithelial tissue of the large intestine. The cytotoxic effects can be neutralized by probiotics such as Bifidobacterium bifidum. Probiotics are viable cells that have beneficial effects on the health of the host. The preventing activity of B. bifid...

Rotavirus (RV) strain SA-11 was studied with respect to its infectivity in MA-104 cell cultures and the effect of concanavalin A (ConA). Receptors for ConA at the surface of MA-104 cell were determined by fluorescence assay and specifically inhibited by D-mannose. The kinetics of virus growth was carried out by plaque assay. Electron microscopy and polyacrylamide gel electrophoresis were used f...

The aim of the study was to characterize bovine viral diarrhoea virus (BVDV) infection in pregnant cows and their foetuses. Blood samples from 64 clinically healthy cows, determined as pregnant after slaughter, and from their foetuses were collected into tubes with and without EDTA. In total 128 blood samples were examined for BVDV antibodies and 128 leukocyte samples for BVDV antigens by direc...

We show that the anti-human immunodeficiency virus agent, 3'-azido-3'-deoxythymidine (AZT), which suppresses infectivity and cytopathic effects of human immunodeficiency virus, also effectively inhibits Epstein-Barr virus (EBV) DNA replication. However, AZT has no effect on four other human herpesviruses: cytomegalovirus, varicella-zoster virus, and herpes simplex virus types 1 and 2. The combi...

Complete inactivation of infectious Ebola virus (EBOV) is required before a sample may be removed from a Biosafety Level 4 laboratory. The United States Federal Select Agent Program regulations require that procedures used to demonstrate chemical inactivation must be validated in-house to confirm complete inactivation. The objective of this study was to develop a method for validating chemical ...

Hinze, Harry C. (University of Wisconsin, Madison), and Duard L. Walker. Variation of herpes simplex virus in persistently infected tissue cultures. J. Bacteriol. 82:498-504. 1961.-Cultures of human conjunctiva, HeLa, and KB cells infected with herpes simplex virus (HF strain) were cultured for prolonged periods in medium containing low levels of antibody. After continuous culture of the infect...

Somerson, Norman L. (National Institute of Allergy and Infectious Diseases, Bethesda, Md.), and M. K. Cook. Suppression of Rous sarcoma virus growth in tissue cultures by Mycoplasma orale. J. Bacteriol. 90:534-540. 1965.-An agent which produced cell destruction in human diploid and chick-embryo fibroblasts was isolated from WI-26 strain of human diploid fibroblasts and shown to be a mycoplasma....