DNA barcoding of the cytochrome oxidase subunit I (COI) gene was utilized to assess the species diversity of the freshwater halfbeak genus Hemirhamphodon. A total of 201 individuals from 46 locations in Peninsular Malaysia, north Borneo (Sarawak) and Sumatra were successfully amplified for 616 base pairs of the COI gene revealing 231 variable and 213 parsimony informative sites. COI gene trees ...

The market for canned mackerel is growing due to their competitive price and the excellent properties of meat. However, weak rules current legislation, coupled with loss discrimination power being obliged use short barcode regions, open up new challenges traceability in processed products. In this study, first time, we applied a two-step mini-barcoding approach identify sold Italian markets mak...

Erynnis tages and marloyi were known as European species until recent years. Due to their narrow distribution areas, the morphological similarities of two very high, status was controversial. However, records these came from new regions, areas turned out be wide, contrary what is known. With mtCOI gene barcode, there a chance identify genetic variations hidden between inter-species intra-specie...

Chironomids are one of the most biodiverse and abundant members freshwater ecosystems. They a food source for many organisms, including fish water mites. The accurate identification chironomids is essential applications in ecological research, determining which chironomid species present diets diverse predators. Larval adult from habitats, lakes, rivers, inland gardens, coastal vegetation, near...

BACKGROUND DNA barcoding has been advanced as a promising tool to aid species identification and discovery through the use of short, standardized gene targets. Despite extensive taxonomic studies, for a variety of reasons the identification of fishes can be problematic, even for experts. DNA barcoding is proving to be a useful tool in this context. However, its broad application is impeded by t...

BACKGROUND DNA barcode differences within animal species are usually much less than differences among species, making it generally straightforward to match unknowns to a reference library. Here we aim to better understand the evolutionary mechanisms underlying this usual "barcode gap" pattern. We employ avian barcode libraries to test a central prediction of neutral theory, namely, intraspecifi...

DNA barcoding remains a challenge when applied to diet analyses, ancient DNA studies, environmental DNA samples and, more generally, in any cases where DNA samples have not been adequately preserved. Because the size of the commonly used barcoding marker (COI) is over 600 base pairs (bp), amplification fails when the DNA molecule is degraded into smaller fragments. However, relevant information...

While previous research has indicated the utility of DNA barcoding in identifying spider species sampled from a localized region, the eff ectiveness of this method over a broader geographic scale and with denser taxon sampling has not yet been extensively considered. Using both new and published data from 1801 individuals belonging to 361 morphospecies, this study examined intraand interspecifi...

In order to improve the efficiency and effectiveness of 2D barcode detection algorithm under complex background, detection algorithm for Data Matrix 2D bar code based on regional features analysis and locating method of line Snake algorithm is presented in this paper. Firstly, the region which contains "Ltype" barcode border is selected by analyzing the features of each region. Secondly, the ba...

Abstract The genus Leptochiton Gray, 1847 is a paraphyletic group of small, pale, and usually deepwater chitons. They lack some the morphological shell characters that are important in systematics Polyplacophora, species identification within this challenging. One complex North Pacific includes at least 11 were historically synonymized or confused with rugatus (Carpenter Pilsbry, 1892). Scrutin...