Many different strategies of high performance liquid chromatographic method development are used today. This review describes a strategy for the systematic development of High performance liquid chromatographic (HPLC) methods. HPLC is an analytical tool which is able to detect, separate and quantify the drug, its various impurities and drug related degradants that can form on synthesis or stora...

An internally standardized method for the determination of 3-methylhistidine in human urine is presented. This methylated amino acid and the chemically analogous internal standard 3-ethylhistidine were isolated from human urine specimens using small columns of cation-exchange resin. Quantification was accomplished by high-performance liquid chromatography using post-column derivatization with o...

Inverse gas chromatography (IGC) represents a unique reRnement of a classic chromatographic method in which an unknown solid material such as a powder, Rbre or Rlm may be characterized. By reversing the traditional role of unknown mobile phases and known reference stationary phases, the gas}solid chromatographic retention processes may be used to characterize an unknown particulate sample by el...

A simple fast and precise reverse phase high performance liquid chromatographic method has been developed for the determination of Primidone Tablets. A symmetry (5μ, 150 x 4 .6mm column) in isocratic mode with methanol: Water (50:50) as mobile phase. The Flow rate is1.0ml/min and effluent is

In vitro protein refolding is still a bottleneck in both structural biology and in the development of new biopharmaceuticals, especially for commercially important polypeptides that are overexpressed in Escherichia coli. This review focuses on protein refolding methods based on column procedures because recent advances in chromatographic refolding have shown promising results.