The enzymatic potential of the cultured plant cells can be employed for bioconversion purposes. Plant enzymes are able to catalyze regio- and stereo-specific reactions, and therefore can be applied for the production of desired substances. The biotransformation of foreign substrates with suspension cells of Peganum harmala was tested with (±) phenylethyl propionate. The callus cultures of Pegan...

The indications and results of cytogenetic analysis of 521 amniotic cell cultures performed at 13-16 weeks of gestation were evaluated in this study. 507 fetuses (97.3%) were cytogenetically normal, 14 (2.7%) had unbalanced karyotypes, and 2 fetuses were found to have major abnormalities, one with anencephaly detected by measurement of alpha -fetoprotein levels in amniotic fluid and ultraso...

Background & Aim: Investigation of suspension cell culture and callus production in Catharanthus roseus. Catharanthus roseus is an herbaceous perennial plant belongs to the family Apocynaceae. C. roseus is important as commercial medicinal plant because of its alkaloidal content. Whole plant contains about 130 alkaloids those are classified as vinca alkaloids...

background: plant cell cultivations are considered as an alternative to agricultural processes for producing valuable phytochemicals (secondary metabolites). the use of plant cell cultures has overcome several inconveniences for the production of secondary metabolites. objective: the essential oils isolated from roots of 24-month-old field grown valerian (valeriana sisymbriifolium) and hairy ro...

poultry spermatogonial stem cells (sscs) have the potential to serve as a model for studying the basic biology of ssc and they can also be used for biotechnological purposes. however, the small number of sscs and the presence of the testicular somatic cells with sscs have limited their applications. therefore, this study was undertaken for the first time to investigate the effect of a serum-fre...

in this study the programmed cell death (apoptosis) of human neutrophilic granulocytes was investigated in the presence and absence of fetal calf serum (fcs), cycloheximide and actinomycin-d. the results show that when fcs is omitted from cultures, apart from a decrease in viability, the percentage of apoptotic cells and dna fragmentation increases. apoptosis is accelerated in serum withdrawal ...

Echium amoenum Fisch. and C.A. Mey. (Boraginaceae) is a very popular medicinal plant which is used as a tonic, tranquillizer, diaphoretic, cough remedy, sore throat and pneumonia in Iran’s traditional medicine. Callus culture of medicinal plants is one of the ways for production of secondary metabolites. In this study, callus culture of E. amoenum and its major secondary metabolite were investi...

Abstract INTRODUCTION No immunotherapy exists for meningiomas. NY-ESO-1 is a potential target because in meningiomas it’s the most frequently expressed Cancer-Testis-antigen. Decitabine hypomethylating agent known to upregulate CT-antigens. We tested effects of decitabine on expression primary and immortalized meningioma cultures, its impact efficacy T-cell-receptor-transduced T-cells(TCRs), un...

a procedure was developed for rapid in vitro germination of linum album seeds by scarification and exposing to gibberellic acid (ga3) and kinetin (kn).  concentrations of podophyllotoxin (ptox) and related aryltetralin lignans α-peltatin, β-peltatin, 5’-demethoxy-6-methoxypodophyllotoxin and 6-methoxypodophyllotoxin (mptox) in l. album fresh plant organs were determined by hplc. a degree of var...