Concentrations of cross-linked fibrin degradation products (XL-FDPs) in plasma, measured by enzyme-linked immunosorbent assays (ELISAs) based on monoclonal antibodies (MAbs) raised against fragment D-dimer of cross-linked fibrin, increase when patients are given fibrinolytic agents. Whether XL-FDPs derive from circulating cross-linked fibrin polymers in plasma, compared with clot-associated fib...

alkylhydroperoxide reductase (ahpc, the 26 kda antigen) is one of the abundant antioxidant enzymes in helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect h. pylori infection in clinical specimens. this study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. five established hybridoma cell lines secreti...

Accurate measurement of insulin-like growth factor (IGF) binding protein-1 (IGFBP-1) is important for precise definition of its physiological roles and potential diagnostic values. Because altered phosphorylation results in altered IGFBP-1 immunoreactivity, current assays may significantly underestimate or fail to detect physiological changes in the IGFBP-1 concentrations. We developed three EL...

An enzyme-linked immunosorbent assay (ELISA) for the detection of Rickettsia typhi antigen in homogenates of pooled or individual laboratory infected fleas is described. The assay uses a double sandwich technique, employing a pool of monoclonal antibodies to capture the antigen and a hyperimmune rabbit serum for antigen detection. Using pools of R. typhi infected Xenopsylla cheopis, Ctenocephal...

The unprecedented spread of highly pathogenic avian influenza virus subtype H5N1 in Asia and Europe is threatening animals and public health systems. Effective diagnosis and control management are needed to control the disease. To this end, we developed a panel of monoclonal antibodies (MAbs) against the H5N1 avian influenza virus (AIV) and implemented an antigen-capture enzyme-linked immunosor...

Rift Valley fever (RVF) virus is an important zoonotic and a potential biothreat agent. This paper describes validation of sandwich and capture enzyme-linked immunoassays (ELISA) based on gamma-irradiated antigens for the detection of RVFV-specific IgG and IgM antibody in humans. Validation data sets derived from testing field-collected sera from Africa (n=2400) were dichotomised according to t...

BACKGROUND Samples from babies exhibiting clinical symptoms suggestive of congenital infection are referred regularly to NICD, New Delhi,, from Government Hospitals located in Delhi and a home for abandoned children (Palna), for the diagnosis of etiological agents like toxoplasma, rubella, CMV and herpes. Blood samples of mothers of most of the affected babies are also received. OBJECTIVE Eva...

Abscisic acid (ABA) was quantitated by enzyme-linked immunosorbent assay (ELISA) in water-stressed leaves from control apple seedlings, and also from apple seedlings treated for 28 days with paclobutrazol ([2RS, 3RS]-1-[4-chlorophenyl]-4,4-dimethyl-2-[1,2,4-triazol-1-yl] pentan-3-ol). The ELISA quantitative estimates were also validated by gas chromatography-electron capture detector and lettuc...

Results All enrolled patients had fever with rash without evidence of vesicles. Majority, (n=29) were adults and 18 were male. Of the 14 (38.9%) that were positive by IgM ELISA for VZV, 5 (35.7%) belonged to the age group of 19-30 years, 4 (28.5%) were < 18 years and 2 (14.3%) in age group 51-60 years. All patients had normal platelet count except 9, who had mildly deranged platelet count (>1.5...

We have developed an immuno-polymerase chain reaction protocol which includes a highly purified streptavidin-DNA conjugate. The protocol comprises standard ELISA methodology and washing buffers together with a real-time PCR read-out system. The conjugate was employed in both indirect and capture assay formats, which can be completed in a single day using standard laboratory equipment. The immun...