Enzymatic browning negatively impacts product quality and shelf-life of packaged fresh-cut lettuce. Metabolite profiles lettuce are affected by the process. The purpose this study was to identify metabolomic marker compounds predict browning, which could be applied discern accessions suited for commercial production industrial breeding programs. Romaine with different susceptibilities were eval...

This study reports novel modified rice protein (MRP)-ammonium shellac (NH4SL) enteric composite coatings on millimeter-sized probiotics pellets to protect the survival of during storage, thermal treatments, and simulated gastrointestinal (GI) digestions. An aqueous MRP solution at pH 7.0–13.0 was dropwise added into an ethanol NH4SL 8.2, mixture significantly affected homogeneity MRP-NH4SL susp...

The complete amino-acid sequence was determined for a rabbit-antibody light chain of allotype b4. The light chain was recovered from approximately 20 g of homogeneous antibody to group C streptococcal carbohydrate, which was obtained from an immunized rabbit. Several enzymic digestions were used to obtain the overlapping peptides necessary for the sequence determination. The L chain has 210 ami...

Among the low molecular weight RNAs which have been shown to be localized to the nucleolus are the 4 S, U3, and 8 S RNAs. Of these, the U3 and 8 S RNAs are hydrogen bonded to nucleolar 28 S RNA. The nucleolar U3 RNA migrates as an electrophoretically homogeneous band on 8% polyacylamide gels but was separated into four bands on 16% gels. Although these bands have similar nucleotide compositions...

OBJECTIVES Shengmaisan (SMS) is a traditional Chinese medicine prescription widely used for the treatment of diverse organs in Korea. The interstitial cells of Cajal (ICCs) are pacemaker cells that play an important role in the generation of coordinated gastrointestinal (GI) motility. We have aimed to investigate the effects of SMS in the ICCs in the mouse small intestine. METHODS To dissocia...

UNLABELLED Progress is reviewed towards the development of a global strategy that aims to extend the sensitivity, dynamic range, comprehensiveness and throughput of proteomic measurements based upon the use of high performance separations and mass spectrometry. The approach uses high accuracy mass measurements from Fourier transform ion cyclotron resonance mass spectrometry (FTICR) to validate ...

There is evidence indicating that stromal proteoglycans are an important functional component of the hematopoietic microenvironment. Proteoglycan synthesis was therefore investigated in the MS3-2A and D2XRII hematopoietic stromal cell lines. These lines differ in their capacity to support hematopoiesis in vitro, D2XRII supporting in vitro hematopoiesis, whereas MS3-2A does not. Cells were label...

The cytochemistry and morphology of interphase and con densed mitotic chromosomes are described in ultrathin-seetioned specimens prepared by freezing and drying, postfixation with protein-sensitive bifunctional reagents of low density, basophile staining with an electron-dense dye lake, and enzy matic digestions. In specimens not subjected to enzymes, interphase chromatinic threads were observe...

Sulfation of glycosaminoglycans and giycopeptides was com pared in baby hamster kidney (BHK) cells and the polyoma virus transformants (PY-BHK). Cells were incubated with | fH ]glucosamine and pSJsulfate, and the labeled glycosaminoglycans and giycopeptides were isolated by digesting the cell membrane fraction with pronase followed by gel filtration. Each type of giycosammoglycan in the void vo...