Since the original description of Helicosporidium parasiticum in 1921, members of the genus Helicosporidium have been reported to infect a wide variety of invertebrates, but their characterization has remained dependent on occasional reports of infection. Recently, several new Helicosporidium isolates have been successfully maintained in axenic cultures. The ability to produce large quantity of...

We conducted a long term (4 week) continuous culture study to measure the chronic effects of UV radiation on the alga, Cryptomonas erosa, using three different fluence rates of UV radiation. We measured carbon allocation into carbohydrate, protein and lipid pools, as well as chlorophyll a concentrations and algal and bacterial density. After 21 days, algal density in the control and lowest UV t...

An axenic culture o f Symphyogyna brongniartii has been initiated from spores on modified Benecke medium. Growth was best on Gamborg B5 medium with 0.3% sucrose, with a doubling time o f 36 days. The culture produced the same terpenoids as plants growing in their natural habitat, perrottetianal A being the major constituent. Also found were bicyclogermacrene, ß-barbatene, 8-selinene, ß-cubebene...

Axenic (germfree) and holoxenic (conventional) rats were given a pectincontaining diet (5 %1 or a pectin-free diet. The diet was not sterilized and the axenic state was maintained by the addition of antibiotics. Pectin modified little or not at all the quantity of bile acids in the small intestine, fecal bile acid excretion and fecal elimination of the sum of cholesterol and bile acids. It simi...

The nematode Caenorhabditis elegans's (CE) successful use in studies of aging is well documented. Cold temperature stress of mixed populations of CE provides a rapid inexpensive means of obtaining three life stage-specific cohorts. Cohorts are obtained in quantities that allow acquisition of replicate metabolite profiles of changes associated with development, aging, and senescence. The fractio...

We investigated the effect of oral immunization on the serum and salivary immunoglobulin levels in axenic and conventional NIH mice. Specific antibacterial and antifungal antibodies were measured by passive hemagglutination, and differences in the immunoglobulin classes were measured by radial immunodiffusion. After an oral immunization regimen with either intact Escherichia coli or Candida alb...

Endophytic microbiomes of healthy seed form a symbiotic relationship with their host. Seed and environment are sources microbes that colonize the developing plant; however, influence each remains unclear. Here, using irradiation combined surface sterilization to generate near-axenic disrupted reduced microbiomes, we contrasted colonization potential soil microbiomes. We hypothesized microbiome ...

Some compounds articulated around a piperazine or an ethylenediamine linker have been evaluated in vitro to determine their activity in the presence of a 3T6 fibroblast cell line and an axenic culture of Pneumocystis carinii, respectively. The most efficient antifungal derivatives, namely N,N'-bis(benzamidine-4-yl)ethane-1,2-diamine (compound 6, a diamidine) and N-(benzamidine-4-yl)-N'-phenylet...