Purpose: To evaluate the profile of in vitro susceptibility of yeasts isolated from cases of primary and espisodic vulvovaginitis to two antifungal agents. Methods: 40 Candida isolates from episodes of vulvovaginal candidiasis were identified by classic methodologies. The susceptibility testing of the in vitro fluconazole and ketoconazole activity against the isolates was accessed by E-test. Re...

Published Ahead of Print 31 January 2007. 10.1128/JCM.02450-06. 2007, 45(4):1322. DOI: J. Clin. Microbiol. Michael A. Pfaller Linda B. Boyken, Shailesh Tendolkar, Jennifer Kroeger and Shawn A. Messer, Daniel J. Diekema, Richard J. Hollis, Filamentous Fungi Posaconazole against Clinical Isolates of Antifungal Susceptibility Testing of Compared to Broth Microdilution for Evaluation of Disk Diffus...

background and objectives : the incidence of fungal infections in immunocompromised patients, especially by candida species, has increased in recent years. this study was designed to identify candida species and determine antifungal susceptibility patterns of 595 yeast strains isolated from various clinical specimens. material and methods : identification of the isolates were determined by the ...

The prevalence of drug resistance has become an important issue in various yeast infections, which have a significant effects on both human animal health. In this study, an attempt has been made to determine susceptibility pattern of two antifungal agents Terbinafine and Ketoconazole against 45 oral and non oral Candida albicans isolates using broth microdilution method. Under in vitro conditio...

The increasing magnitude of antifungal resistance as well as the advent of new antifungal drugs has generated a renewed interest in fungal susceptibility testing. We used a previously described disk diffusion method to evaluate the susceptibility profile of a large collection of recent clinical Candida spp. isolates against fluconazole. A total of 1,784 yeast isolates were tested, including the...

Poor susceptibility of Cryptococcus neoformans to fluconazole (FLC) is a matter of concern among clinicians in Africa. The emergence of resistance to FLC was recently reported in Kenya, but it is not known whether it is widespread. Thus, there is need for more antifungal drug susceptibility studies in Kenya. The aim of this study was to measure the in vitro antifungal drug susceptibilities of i...

Objective: Dermatomycosis is an infection with fungi related to the skin: glabrous skin, hair and/or nails. Oral treatment of fungal infections in dermatology has become a preferred modality for the management of these very common conditions. Although there are increasing numbers of antifungals available for treatment of dermatophytes, some cases and relapses have been unresponsive to treatment...

Candida is an opportunistic pathogen commonly found in hospital settings. During recent years increase in the non albican species of Candida and their resistance against treatment of choice has been observed. The aim of this research was to study the prevalence and antifungal susceptibility pattern of Candida species from different hospital units. A total of 303 samples were included in this st...

There is no ideal laboratory procedure or culture medium in current use for susceptibility testing of pathogenic yeasts. Six candidate growth media (RPMI 1640 with L-glutamine, yeast nitrogen base, Casamino Acids medium, Mueller-Hinton broth, Sabouraud dextrose broth, and minimum essential medium-Eagle salts) were screened by spectrophotometric absorbance for nucleic acid and protein. From thes...

A standardized reference method for dermatophyte in vitro susceptibility testing is lacking. In a previous study, Norris et al. (H. A. Norris, B. E. Elewski, and M. A. Ghannoum, J. Am. Acad. Dermatol. 40(6, part 2):S9-S13) established the optimal medium and other growth variables. However, the earlier study did not address two issues: (i) selection of an optimal medium for conidial formation by...