alkylhydroperoxide reductase (ahpc, the 26 kda antigen) is one of the abundant antioxidant enzymes in helicobacter pylori and seems to have a good potential for use in development of immunoassays to detect h. pylori infection in clinical specimens. this study aimed to investigate some properties of this antigen by the produced monoclonal antibodies. five established hybridoma cell lines secreti...

A four-year survey (2012–2015) was carried out to examine the genetic diversity of cucumber mosaic virus (CMV) isolates infecting tomato, as well presence and their satellite RNAs (satRNAs), collecting a total 226 samples throughout most important growing regions in Serbia. Besides CMV-like symptoms, collected also exhibited more severe such systemic necrosis leaves, branches stems, accompanied...

Aims: In this study, it was aimed to investigate the occurrence of cucurbit yellow stunting disorder virus (CYSDV), chlorotic yellows (CCYV) and beet pseudo-yellows (BPYV) in cucumber, zucchini melon plants grown Hatay province by Double Antibody Sandwich-Enzyme Linked Immuno Sorbent Assay (DAS-ELISA) Reverse Transcription-Polymerase Chain Reaction (RT-PCR) methods, analyse phylogenetic relatio...

Changes in the mass and subunit structure of liver acetyl-CoA carboxylase (ACC) accompany altered nutrition in vivo. Enzyme activity in different tissues and cell lines is also, in part, determined by variations in both total mass and ACC isoenzyme composition. ACC isoenzyme mass and hetero/homo-isoenzyme association were quantified by three sandwich e.l.i.s.a. assays, i.e. an avidin-based assa...

Clostridium perfringens type A enterotoxin was specifically detected and readily quantified by indirect and four-layer sandwich enzyme-linked immunosorbent assays (ELISAs). With the indirect ELISA, enterotoxin was detected in quantities of as low as 2.5 ng (25 ng/ml). When the more sensitive sandwich ELISA procedures was used, 100 pg (1 ng/ml) of enterotoxin was detected. The sandwich ELISA pro...

The chemiluminescence of fluorescein derivatives is greatly enhanced when they are conjugated to proteins. We exploited this property in devising a chemiluminescence immunoassay for rubella antibodies. In this sequential "sandwich"-type assay, tubes coated with rubella antigen are used for antibody separation and goat antibody to human IgG conjugated with fluorescein isothiocyanate is used for ...

Research on Parkinson’s disease (PD) has been focused the development of PD diagnostic tools as much therapeutics. Several genetic culprits PD, including DJ-1, Leucine-rich repeat kinase 2 (LRRK2), and ?-synuclein (?-syn), have investigated markers in human biofluids. Unfortunately, approaches to develop are impractical, there is a considerable demand for an appropriate marker PD. The measureme...