Intestinal trefoil factor (ITF or TFF3), NO and epithelium-associated mucin have important roles in sustaining mucosal integrity in the gastrointestinal tract. In the present study we examined ITF-binding molecules on IEC-18 cells (an intestinal epithelial cell line) with the use of flow cytometry and localized these molecules on the cell surface by confocal microscopy. Furthermore, we studied ...

Interfacial properties of two brush-with-anchor mucins, C-P55 and C-PSLex, have been investigated at the aqueous solution/poly(methyl methacrylate) (PMMA) interface. Both are recombinant mucin-type fusion proteins, produced by fusing the glycosylated mucin part of P-selectin glycoprotein ligand-1 (PSLG-1) to the Fc part of a mouse immunoglobulin in two different cells. They are mainly expressed...

The use of snail mucin motifs as rectal absorption enhancer for insulin has been evaluated. The mucin motifs were extracted from the giant African snail Archachatina marginata by differential precipitation with acetone. The mucin motifs were found to have a molecular weight of 5780 Da and an isoelectric point of 3.4. At the concentrations evaluated, the mucin exhibited rectal absorption enhanci...

We have studied the biosynthesis of mucin glycoproteins recognized by monoclonal antibody W1 in MCF7 cells. Proteins of Mr 170,000, 185,000, 260,000, and 275,000 were immunoprecipitated from cells pulse-labeled with [3H]threonine and [3H]proline. Evidence suggesting that these proteins were precursors of high molecular weight mucin(s) included: 1) their kinetics of disappearance corresponded wi...

The effects of mucin on cholera toxin induced intestinal secretion was studied in the rat small intestine. Gastric mucin inhibited secretion when premixed with cholera toxin on an equal dry-weight basis. Neither salivary nor intestinal mucin inhibited the toxin's action in the intestine. Inhibition by gastric mucin was not reversed by a mucolytic agent or by hexoses or agents that bind hexoses....

Colorectal carcinoma is a major cause of cancer associated with a high rate of morbidity and mortality in the western world. One of the pathologic features considered to be important for prognostic is mucin production. Many authors confirmed that colon carcinomas with high mucin content tend to re-occur locally and carry a poor prognosis. For histochemical evaluation of mucin content, we invest...

AIMS Gastric hyperplastic polyps (GHPs) are the most common polypoid lesion of the stomach, and their malignant potential has been demonstrated. In the present study, we evaluated the mucin phenotypes of GHPs and investigated the relationships among mucin phenotypes and clinical-pathological factors, proliferative activity and p53 expression in GHPs. METHODS The CD10, MUC2, MUC5AC and MUC6 ex...

The cause of the mucus clearance problems associated with cystic fibrosis remains poorly understood though it has been suggested that mucin hypersecretion, dehydration of mucins, and biochemical abnormalities in the glycosylation of mucins may be responsible. Since the biochemical and biophysical properties of a mucin are dependent on O-glycosylation, our aim was to evaluate the O-glycosylation...

Late-gestation (embryonic day 18; E18) mouse submandibular glands (SMG) comprise a network of large and small ducts that terminate in lumen-containing, presumptive acini (terminal buds) expressing unique, cell membraneassociated embryonic mucin. The objective here was to clone and sequence embryonic low molecular-weight SMG mucin, predict its secondary structure, and begin to investigate its po...

Background and Objectives:Histochemical studies have shown that differentiated gastric cancer(D-GC)can be classified according to the phenotype of expressed mucin.In the present study, we investigated the biological divergence of early stage D-GCs expressing different types of mucin. Methods:Specimens from 73 surgically resected early stage D-GCs were classified on the basis of the phenotype of...