The central metabolic enzyme fructose-1,6-bisphosphate aldolase (Fba1p) catalyzes a reversible reaction required for both glycolysis and gluconeogenesis. Fba1p is a potential antifungal target because it is essential in yeast and because fungal and human aldolases differ significantly. To test the validity of Fba1p as an antifungal target, we have examined the effects of depleting this enzyme i...

The gene encoding the Escherichia coli Class I fructose-1, 6-bisphosphate aldolase (FBP aldolase) has been cloned and the protein overproduced in high amounts. This gene sequence has previously been identified as encoding an E. coli dehydrin in the GenBanktrade mark database [gene dhnA; entry code U73760; Close and Choi (1996) Submission to GenBanktrade mark]. However, the purified protein over...

No. st-j476 Rabbit Muscle Aldolase catalytic mechanism: Trapping of covalent intermediates M. St-Jean, J. Sygusch (Université de Montréal) Beamline(s): X8C Introduction: Rabbit muscle aldolase (EC 4.1.2.13) is a class I fructose-1,6-bisphosphate aldolase that utilizes covalent enzymatic intermediates in its catalytic mechanism [1]. Although the chemical composition of these covalent intermediat...

tial element for more than a hundred years, ever since it was discovered by Raulin in 1869 to be required for the growth of Aspergillus niger. However, it has not been until relatively modern times that the universal importance of this metal for both human and animal health became fully appreciated. To date more than 200 zinc dependent enzymes have actually been identified in all the main bioch...

The catalytic and immunochemical properties of homomerit and heteromeric rabbit fructose diphosphate aldolase tetramers are described. Heteromers composed of either A and C (A&!, A&, AC) or B and C (B&Z, B&, BCJ subunits have maximal velocities and Michaelis constants indistinguishable from those of an equivalent mixture of homomers. These results indicate the subunits act independently and als...

Mouse monoclonal antibody 38C2 is the prototype of a new class of catalytic antibodies that were generated by reactive immunization. Through a reactive lysine, 38C2 catalyzes aldol and retro-aldol reactions using the enamine mechanism of natural aldolases. In addition to its remarkable versatility and efficacy in synthetic organic chemistry, 38C2 has been used for the selective activation of pr...

nm23 genes have been implicated in the suppression of tumour metastasis and cell motility; however, the biochemical mechanisms for these suppressions are not known. We have previously described the transfer of phosphate from the catalytic histidine residues of nm23 proteins to an aspartic or a glutamic residue on one or more 43 kDa proteins in detergent extracts of bovine brain membranes. To ga...

The kinetic parameters of the 10 glycolytic enzymes and glycolytic fluxes were determined for the first time in Ustilago maydis. Enzyme activities in yeast grown in minimal medium and harvested in the stationary stage were twofold higher than those from yeast grown in rich medium. In contrast, in yeast harvested in the exponential stage, the enzyme activities were higher in cells grown in rich ...

Both the Schiff base-mediated 2-keto-4-hydroxyglutarate aldolase of liver and the metal-activated a-keto-/3-deoxyhexarate aldolase of Escherichia coli condense pyruvate and glyoxylate to form (4-RS)-Z-keto-4-hydroxyglutarate. Both enzymes catalyzed a condensation between [3-3H3]pyruvate and glyoxylate. It was found that C-C synthesis occurred 6 to 7 times faster than C-T bond cleavage. Thus, py...

SnoaL belongs to a family of small polyketide cyclases, which catalyse ring closure steps in the biosynthesis of polyketide antibiotics produced in Streptomyces. Several of these antibiotics are among the most used anti-cancer drugs currently in use. The crystal structure of SnoaL, involved in nogalamycin biosynthesis, with a bound product, has been determined to 1.35 A resolution. The fold of ...