Adipocytes are a significant component of the bone marrow microenvironment. Although bone marrow adipocytes were first identified more than 100 years ago, it is only in recent years that an understanding of their complex physiological role is emerging. Bone marrow adipocytes act as local regulators of skeletal biology and homeostasis, with recent studies suggesting that marrow adipose tissue is...

Adipose (fat) tissues contain white, brown, and beige adipocytes. White adipocytes store extra energy in the form of lipids; ectopic and excessive accumulation of white adipose in the body leads to overweight and obesity, which often causes insulin resistance and Type 2 diabetes. Brown adipocytes, in contrast, contain hundreds of mitochondria per cell, dissipate energy into heat, and are negati...

Adipocytes promote cancer progression and impair treatment, and have been shown to protect acute lymphoblastic leukemia (ALL) cells from chemotherapies. Here we investigate whether this protection is mediated by changes in oxidative stress. Co-culture experiments showed that adipocytes protect ALL cells from oxidative stress induced by drugs or irradiation. We demonstrated that ALL cells induce...

Energy homeostasis is regulated by peripheral signals, such as leptin, and by several orexigenic and anorectic neuropeptides. Recently, we reported that the orexigenic neuropeptide melanin-concentrating hormone (MCH) stimulates leptin production by rat adipocytes and that the MCH receptor (MCH-R1) is present on these cells. Here, we show that MCH-R1 is present on murine 3T3-L1 adipocytes. Treat...

Apolipoprotein E (apoE) is highly expressed in adipose tissue and adipocytes in which its expression is regulated by peroxisome proliferator-activated receptor (PPAR)-gamma agonists and tumor necrosis factor-alpha. There is, however, no information regarding a role for endogenous apoE in differentiated adipocyte function. In this report, we define a novel role for apoE in modulating adipocyte l...

The present study was undertaken to provide further information on lipogenesis in isolated adipocytes from the subcutaneous adipose tissue of fattening steers. The main aims were to compare different incubation media and to clarify the effects of fetal bovine serum (FBS) on lipogenesis and substrate oxidation rate in the adipocytes. The isolated adipocytes were prepared by the collagenase diges...

The activity, content, and turnover of the key lipogenic enzyme fatty acid synthetase were studied during the differentiation of 3T3-Ll preadipwytes into adipocytes. The specific activity of the enzyme began to rise sharply when triglyceride droplets were first detected in the cells (Day 3) and ultimately increased 13-fold to a new steady state level in adipocytes 7 days after the initiation of...

Nitric oxide (NO) has been recognized as a potential mediator of inflammation-induced metabolic alterations, including insulin resistance. However, expression mechanisms and potential roles of endothelial and inducible NO synthases (eNOS and iNOS, respectively) in human adipocytes are poorly understood. In the present study, we aimed to analyze several aspects of NO-related gene expression and ...

Abstract Isoacteoside is a caffeoyl phenylethanoide glycoside found in various plant parts, such as the flower of Magnolia denudata . In particular, magnolia has been studied for its anti-obesity, anticancer, and anti-inflammatory effects. However, isoacteoside not extensively studied, except this study, anti-obesity effects were investigated 3T3-L1 mouse pre-adipocytes. treatment did induce cy...