نتایج جستجو برای: adenylate cyclase toxin
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We read, with considerable dismay, a recent Research Article on cholera toxin (CT) internalization (Torgersen et al., 2001), in which the authors extensively challenged methods, results and conclusions that we had published four years ago (Orlandi and Fishman, 1998). As space limits a point-by-point rebuttal of their comments and critique of the many deficiencies in their study, we encourage re...
A guanosine 5'-[gamma-[35S]thio]triphosphate-binding activity was detergent-extracted from Trypanosoma cruzi membranes. This binding activity was co-eluted from gel-filtration columns with a factor which, in a heterologous reconstitution system, blocks glucagon stimulation of adenylate cyclase activity in liver membranes. ADP-ribosylation of these membranes by pertussis toxin eliminated this bl...
Dopamine-sensitive adenylate cyclase was reconstituted from the cholate-soluble components of caudate nucleus homogenate. Biological function was restored by precipitating the components from cholate and phospholipid with ammonium sulfate, dialyzing the resuspended precipitate, and activating the particulate complex with phospholipid in the assay. The reconstituted adenylate cyclase was stimula...
The effect of rat synthetic atrial natriuretic factor (ANF) on adenylate cyclase activity was studied in adrenal cortical membranes. Synthetic ANF (Arg 101-Tyr 126) inhibited adenylate cyclase activity in a concentration-dependent manner. The maximum inhibition observed was about 25% with an apparent ki of 5 X 10(-11) to 10(-10) M. Various hormones such as isoproterenol, dopamine, prostaglandin...
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