The present work dealt with RAPD markers for genetic diversity comparison estimation in twenty three cotton varieties of Bt and non-Bt cotton cultivars belonging to two cultivated species of cotton in Andhra Pradesh state, India.Variations among twenty three different cotton varieties were analyzed with selected 40 RAPD primers using the polymerase chain reaction (PCR). All the primers produced...

In the present work, strain-specific PCR primers for Lactobacillus rhamnosus Lc 1/3 are described. The randomly amplified polymorphic DNA (RAPD) technique was used to produce potential strain-specific markers. They were screened for specificity by hybridization with DNA from 11 L. rhamnosus strains. A 613-bp RAPD marker found to be strain-specific was sequenced, and a primer pair specific to L....

Abstract Fusarium head blight (FHB) is consistently one of the most important barley diseases worldwide. This study aimed to evaluate pathogenicity 16 isolates four species under controlled conditions and their genetic variability using 22 random amplified polymorphic DNA (RAPD) markers. Pathogenic variation was characterized based on disease development rates index two Syrian landraces with va...

Numerous methods have been developed for direct sequencing of single or double-stranded DNA amplified by PCR, and most require template purification steps prior to sequencing to remove excess unincorporated primers and dNTPs. Purification methods can be cumbersome, laborious and time consuming, or yield low DNA recoveries. Strategies for direct sequencing of doublestranded DNA products without ...

The randomly amplified polymorphic DNA (RAPD) technique was used in the development of a fingerprinting (typing) and identification protocol for Legionella strains. Twenty decamer random oligonucleotide primers were screened for their discriminatory abilities. Two candidate primers were selected. By using a combination of these primers, RAPD analysis allowed for the differentiation between all ...

The genetic diversity of 88 Streptococcus suis serotype 2 isolates which were recovered from various countries was examined by randomly amplified polymorphic DNA (RAPD) analysis with three primers. This bacterial collection included 80 isolates of porcine origin and 8 of human origin. This investigation allowed the identification of 23 RAPD types containing 1 to 30 isolates originating from one...

The pomegranate has been used traditionally in Coruh Valley in Turkey for a long time; fruits are harvested from wild, semi-domesticated and cultivated trees. In the valley, it occurs in general along with olive trees. We sampled 23 wild-grown pomegranate genotypes sampled from different parts of Coruh Valley and compared them using RAPD primers to determine genetic variability. Eighty-six RAPD...

We present protocols and conditions for specimen storage, DNA extraction and storage, and the subsequent RAPD (Random Amplified Polymorphic DNA) profiling of spiders . Three common UK species, Lepthyphantes tenuis (Blackwall 1852), Enoplognatha ovata (Clerk 1757) and Clubiona reclusa (Cambridge 1863), members of the Linyphiidae, Theridiidae and Clubionidae respectively, were chosen to serve as ...

Cenchrus is an important component of major grass cover of world. Similar to the other major tropical grasses most of the species in genus Cenchrus are also apomictic in nature hence correct and precise identification of accessions and species are problematic and dubious. In the present study 187 decamer oligonucleotide primers were tested for PCR-based DNA amplification of six prominent specie...