As a part of our programme to develop nanobioconjugates for the treatment of cancer, we first synthesized extracellular, protein-capped, highly stable and well-dispersed gadolinium oxide (Gd2O3) nanoparticles by using thermophilic fungus Humicola sp. The biodistribution of the nanoparticles in rats was checked by radiolabelling with Tc-99m. Finally, these nanoparticles were bioconjugated with t...

with amperometric detection. It has been shown elsewhere that the selectivity of this method is sufficient to separate the potential organic impurities; that is, synthesis byproducts and a specified degradation product from the drug substance (3). The objective of this article is to demonstrate that this method’s suitability for stability testing can be evaluated easily by performing HPLC with ...

General chemical synthesis methods. Anhydrous solvents were obtained commercially. Solvents required for oxime ligation coupling reactions were distilled from 2,4-dinitrophenylhydrazine immediately prior to use. Air-sensitive reactions were carried out under Ar. Thin layer chromatography (TLC) was performed on aluminium sheets coated with 60 F254 silica. Flash column chromatography was performe...

The development of a sensitive automated method for residue control of oxytetracycline (OTC) in cured meat (soudjouk) is described. The principle steps involve the extraction of OTC from cured meat in the presence of citric acid with nitric acid, methanol and de-ionized water, and then the determination by HPLC. In HPLC, Hypersil BDC C18 column was used and OTC was separated at 24◦C using a mob...

Isolation and purification of the active principle within natural compounds plays an important role in drug development. MS (mass spectrometry) is used as a detector in HPLC (high performance liquid chromatography) systems to aid the determination of novel compound structures. Clustering techniques provide useful tools for intelligent data analysis within this context. In this paper, we analyse...

Metabolite identification is a key step for metabonomics study. A fully automated hyphenation of HPLC-diode-array detector (DAD) mass spectrometry (MS) solid phase extraction (SPE)-NMR spectroscopy (HPLC-DAD-MS-SPE-NMR) is one of the most efficient methods to determine the structure of a given unknown metabolite in a complex mixture (metabonome) and hence represents one of the most important an...

A column-switching high-performance liquid chromatography (HPLC) system for the determination of phosphopeptides has been developed. The method is based on the selective adsorption of phosphopeptides on a titania (TiO2) precolumn and successive HPLC separation of the phosphopeptides on an anion-exchange column with a UV detector (215 nm). The recoveries of phosphopeptides were tested using auth...

Two multivariate calibration methods are compared for the simultaneous chromatographic determination and separation of Sulfamethoxazole (SMX) and Phthalazine (PHZ) by High Performance Liquid Chromatography (HPLC). Multivariate calibration techniques such as Classical Least Squares (CLS) and Inverse Least Squares (ILS) were introduced into HPLC to determine the quantification by using UV detecto...

An accurate, reproducible and sensitive method for the quantitative determination of sarcophine in the organic extract of the Red Sea soft coral Sarcophyton species was developed and validated. Sarcophine concentration was determined by RP HPLC using ODS column. The mobile phase was made up of 70% acetonitrile in deionized water and the pH was adjusted to 3.5 with phosphoric acid. The flow rate...