Statistically designed experiments were used to optimize the production of arginine deiminase (ADI) by Pseudomonas putida KT2440 in batch culture. A Plackett-Burman design involving eleven factors showed that ADI production was most influenced by the initial pH and the initial concentrations of glucose and yeast extract. A central composite experimental design showed that the optimal values of ...

Lactococcus lactis is highly efficient probiotics microorganism with wide range of benefits on human health. This study was conducted to design and establish industrial platform for high cell density cultivation of this novel probiotic strain, L. lactis (WICC-B25). During bioreactor cultivation of lactic acid bacteria in industrial scale, there are two main problems during process namely: low c...

BACKGROUND The eukaryotic RNase ribonuclease/angiogenin inhibitors (RI) are a protein group distinguished by a unique structure - they are composed of hydrophobic leucine-rich repeat motifs (LRR) and contain a high amount of reduced cysteine residues. The members of this group are difficult to produce in E. coli and other recombinant hosts due to their high aggregation tendency. RESULTS In th...

Insufficient mixing in large-scale bioreactors provokes gradient zones of substrate, dissolved oxygen (DO), pH, and other parameters. E. coli responds to a high glucose, low feeding zone with the accumulation mixed acid fermentation products, especially formate, but also synthesis non-canonical amino acids, such as norvaline, norleucine ?-methylnorleucine. These acids can be mis-incorporated in...

The production of butanol, acetone and ethanol by Clostridium acetobutylicum is a biphasic fermentation process. In the first phase carbohydrate substrate metabolized to acetic butyric acid, in following second product spectrum shifted towards economically interesting solvents. Here we present cascade six continuous stirred tank reactors (CCSTR), which allows performing time dependent metabolic...