Background Myeloid-derived suppressor cells (MDSCs) are heterogeneous immature myeloid with suppressive functions (1). It is known that MDSCs expanded in inflammatory sites after migrating from bone marrow (BM) or spleen. Rheumatoid arthritis (RA) an autoimmune disease characterized as polyarthritis. Although previous reports indicate increased BM and spleen of model mice, detailed analysis joi...

This study was carried out to develop and characterize mouse monoclonal antibodies (mAbs) against chicken CD25 (chCD25), the alpha chain of the interleukin-2 (IL-2) receptor. A recombinant chimeric chCD25/IgG4 fusion protein was expressed in Chinese hamster ovary (CHO) cells and isolated from spent cell culture medium by protein G affinity chromatography. Purified chCD25 protein was used to imm...

The HOX11 homeobox gene was identified via the translocation t(10;14) in T cell leukaemia. To determine the function of this gene in mice, null mutations were made using homologous recombination in ES cells to incorporate lacZ into the hox11 transcription unit. Production of beta-galactosidase from the recombinant hox11 allele in +/- mutants allowed identification of sites of hox11 expression w...

The functional role of the T cell (Tv) which can replicate vesicular stomatitis virus (VSV) on activation by the antigen was investigated in antibody response in vitro. By the inoculation of VSV into the culture, marked augmentation of antibody response to sheep erythrocytes (SRBC) was observed in the culture of spleen cells taken more than 3 days after the immunization with SRBC, suggesting th...

In a study of the nucleic acid content of isolated nuclei, the average desoxypentosenucleic acid (DNA) content of nuclei from transplanted leukemic spleen (line 9421) was found to be increased to 1.45 times the normal value (6). Since only 5-10 per cent of the nuclei were recovered, however, the possibility arose that these nuclei were not repre sentative of the total population. Since all the ...

Hematopoietic efficacy in vivoof multiple injections of purified murine I -cell and recombinant human macrophage colony-stimulating factors (M-CSF; specific activity, >2 x IO7 units/mg) was assessed in mice. Injections i.v. of sterile saline or 20,000 units of M-CSF were adminis tered once (at 0 h), twice (at 0 and 12 h), or three times (at 0, 12, and 24 h) to C57BL/6 x DBA/2 F, mice. Numbers a...

In a study of the nucleic acid content of isolated nuclei, the average desoxypentosenucleic acid (DNA) content of nuclei from transplanted leukemic spleen (line 9421) was found to be increased to 1.45 times the normal value (6). Since only 5-10 per cent of the nuclei were recovered, however, the possibility arose that these nuclei were not repre sentative of the total population. Since all the ...

Hematopoietic efficacy in vivo of multiple injections of purified murine L-cell and recombinant human macrophage colony-stimulating factors (M-CSF; specific activity, greater than 2 x 10(7) units/mg) was assessed in mice. Injections i.v. of sterile saline or 20,000 units of M-CSF were administered once (at 0 h), twice (at 0 and 12 h), or three times (at 0, 12, and 24 h) to C57BL/6 x DBA/2 F1 mi...

We have previously shown that the combination of allogeneic intra-bone marrow-bone marrow transplantation (IBM-BMT) and donor lymphocyte infusion (DLI) using CD4+ cell-depleted spleen cells is effective in suppressing tumor growth, but that this does not induce graft-versus-host disease (GVHD) in mice. In this report, we show that formalin-fixed tumor cell-pulsed dendritic cells (FFTCP DCs) hav...

discussion splenic artery aneurysm is a very uncommon cause of page kidney and to our knowledge, it was the first case of its kind ever reported in the literature. introduction the page kidney is a rare phenomenon that refers to hypertension resulting from any external compression of a kidney by a hematoma, tumor, lymphocele, or urinoma. the activation of the renin-angiotensin-aldosterone syste...