The influence of various technical procedures and diluent media upon human spermatozoa has been tested in vitro. The percentage and velocity of motile spermatozoa were measured objectively using laser Doppler velocimetry. Vitality was determined by fluorescent staining. Centrifugation of semen (diluted 1 : 1 with Tyrode) at forces of 500, 800, 1 800 and 2 500 X g resulted in almost complete sed...

Changes were studied in induced photon emission (as an indicator of oxidative stress), ATP level and sperm motility during seven day-storage of boar semen at 15 degrees C extended with the use of BTS extender. Photon emission was measured using a luminometer equipped with a cooled photomultiplier with a spectral response range from 370 to 620 nm. The time of storage had a significant effect on ...

The purpose of this study was to investigate whether sperm selection by hyaluronic acid (HA) binding could improve fertilization rate and embryo quality in intracytoplasmic sperm injection (ICSI) cycles. Two hundred nineteen oocytes obtained from eighteen women were injected with either HA-bound (n = 107) or conventionally selected spermatozoa (n = 112) in a randomized way. All of the participa...

This study investigated the concentration/time dependent effects of nanosilver colloid on the motionparameters of the ejaculated ram spermatozoa in vitro. After incubation of sperm samples for 30, 60, 120 and 180 min in the presence of nanosilver colloid (0, 0.001, 0.01, 0.1, 1 and 10 ppm), the motion parameters were evaluated by computer assisted sperm analysis. There was a significant (P

Spermatid specific thioredoxin-3 (SPTRX3 or TXNDC8) is a testis/male germ line specific member of thioredoxin family that accumulates in the superfluous cytoplasm of defective human spermatozoa. We hypothesized that semen levels of SPTRX3 are reflective of treatment outcome in assisted reproductive therapy (ART) couples treated by in vitro fertilization (IVF) or intracytoplasmic sperm injection...

Sixty semen ejaculates from 10 mature bulls, 5 each of Jafarabadi and Mehsana breed, were studied for sperm motility and velocity parameters of fresh and frozen-thawed spermatozoa using computer assisted sperm analyzer (CASA). The mean values of motile and progressively motile spermatozoa observed in fresh semen of Jafarabadi and Mehsana bulls (79.77±1.62 and 61.80±1.85, and 78.90±1.22 and 61.3...

In an earlier work done in our laboratory, we have been able to isolate a sperm agglutinating strain of Escherichia coli from the semen sample of a male attending infertility clinic. Further, factor responsible for sperm agglutination (SAF) was isolated and purified, and, using SAF as a tool, corresponding SAF binding receptor from human spermatozoa has been purified. Characterization of SAF an...

Background: Detection of sperm parameters based on conventional methods, immediately after thawing, have been revealed sperm capacity to in vivo and in vitro fertilization. But this evaluation is not fully reliable because of long journey of sperm during female genital tract to reach the oocyte and fertilize it. Therefore, analyzing postthawed sperm parameters after incubation is more beneficia...

BACKGROUND Recently, motile sperm organelle morphology examination (MSOME) criteria as a new real time tool for evaluation of spermatozoa in intracytoplasmic sperm injection (ICSI) cycles has been considered. OBJECTIVE The aim was to investigate the predictive value of MSOME in in vitro fertilization (IVF) in comparison to ICSI cycles and evaluation of the association between MSOME parameters...

in this study, three different age groups of male broodstocks (4, 5 and 6 years respectively) were used to fertilize eggs obtained from nine females. the results showed that 6 year old males had maximum body weight (1766 g), total length (56.3 cm) and sperm volume (31.83 ml). results did not show significant difference in spermatocrit and spermatozoa concentration among age groups (p>0.05). our...