The reactions of anti-I cold-agglutinin antibodies with the red cells from normal adults (adult-I) and adult-i donors and from newborn infants have been investigated by the use of the C’la Fixation and Transfer Test of Borsos and Rapp. Ag. glutination and high concentration of serum reduce the amounts of the C’la fixation by these antibodies. Inhibition by serum is due to an inhibitor of C’la. ...

Increased levels of hypo-galactosylated immunoglobulin (Ig)A1 (HG-IgA1) in IgA nephropathy (IgAN) have been detected using a Helix aspersa agglutinin lectin enzyme-linked immunosorbent assay (ELISA). In this study, we developed monoclonal antibodies to evaluate the HG-IgA1 in IgA nephropathy, aiming to gain a more consistent and reproducible assay. As an analogue to the HG-IgA1 hinge region, a ...

methods this prospective observational study was conducted in a tertiary level neonatal intensive care unit, princess esra hospital, deccan college of medical sciences, hyderabad, telangana, india. consecutive intramural and extramural neonates admitted to neonatal intensive care unit with the rh-negative mother’s blood group and hyperbilirubinemia were enrolled. neonates born to rh+ve mothers ...

The role of sialic acid in the interaction of sialoglycoproteins with wheat germ agglutinin was investigated by using several well characterized saccharides and sialoglycoconjugates. N-Acetylneuraminic acid and neuramin 2 + 3 lactose, in addition to N-acetyl-Dglucosamine and its fil-+ 4 oligomers were found to be inhibitors of wheat germ agglutinin-induced hemagglutination. Neuraminic acid-/?-m...

The influence of varying the amount of wheat germ agglutinin immobilized on Sepharose beads on the binding of glycoproteins to these beads was investigated. A series of wheat germ agglutinin-Sepharose gels containing between 0.10 and 10.0 mg of lectin/ml of gel was prepared, and the actual lectin content was established by acid hydrolysis of the gel followed by analysis of glycine, a major amin...

The value of a quantitative approach to serum therapy of type b H. influenzae meningitis has been established by the experience of a number of institutions. It is recognized that whereas some patients respond promptly following the administration of 50 mg. of specific type b H. influenzae antibody nitrogen others need several hundred milligrams before recovery from infection is complete. The re...

A 57-year-old woman presented in November 1978 with a two-month history of intermittent fever, an evanescent non-pruritic rash, loss of 10 kg, and generalised lymphadenopathy and hepatosplenomegaly. Haemoglobin concentration was 7 8 g/dl, white cell count 316 x 109/1 (31 600/mm3), and platelet count 142 x 109/1. Erythrocyte sedimentation rate was 102 mm in first hour (Westergren), and Coombs te...

The carbohydrate content of purified Bacillus thuriniensis subsp. israelensis crystal toxin was determined by six biochemical tests, column chromatography on an amino acid analyzer, and the binding of 11 fluorescent lectins. The crystals contained approximately 1.0% neutral sugars and 1.7% amino sugars. The amino sugars consisted of 70% glucosamine and 30% galactosamine. No N-acetylneuraminic a...

Activation of the classical pathway (CP) of complement is often associated with autoimmune disorders in which disease pathology is linked to the presence of an autoantibody. One such disorder is cold agglutinin disease (CAD), an autoimmune hemolytic anemia in which autoantibodies (cold agglutinins) bind to red blood cells (RBCs) at low temperatures. Anemia occurs as a result of autoantibody-med...