Collagen and glycosaminoglycans (GAGs) constituting the ECM may limit the space available and thus exclude macromolecules from a fraction of the interstitial fluid (IF) phase. This exclusion phenomenon is of importance for transcapillary fluid and solute exchange. The purpose of the study was to examine the range of interstitial exclusion in rat skin by using probes within a span of molecular w...

ABSTRACT-This experiment was carried out as factorial based on a randomized complete block design with three replications at the research station of the Islamic Azad University-Arak Branch, Iran, in 2011 and 2012. Experimental factors consisted of three levels of salicylic acid (SA); seed priming with distilled water (hydro priming), seed priming with 0.5 mM SA, and 0.5 mM SA  seed priming 1 mM...

Selenium (Se) is considered as an essential element for human body. Selenized probiotic fermented milk drink (DOOGH) as a functional beverage to increase Se available in body. The predominate sugar, protein and mineral ion of Iranian yoghurt drink (DOOGH) are lactose, casein and calcium, respectively. Different levels of  Selenium (2.5 – 3- 3,5-  4 and 4.5) is prepared and its suitability based...

Absorption spectrophotometry has been and still is the industry standard for detection in HPLC. Limit of detection (LOD) and linear dynamic range (LDR) are the primary performance requirements and have driven continuous improvement of spectrophotometric HPLC detectors. Recent advances in HPLC column technology have led to low flow-rate HPLC such as capillary HPLC and nanoflow HPLC and put highe...

Otimizou-se método para monitoração plasmática de bussulfano em pacientes submetidos a transplante células-tronco hematopoiéticas (TCTH). Utilizou-se HPLC-PDA Shimadzu, coluna C18 (150 mm x 4 mm), metanol/água/acetonitrila (65:20:15, v/v/v), fluxo 1 mL min-1; UV λ = 276 nm, tempo análise 17 min; derivatização com dietilcarbamato sódio, extração acetato etila; curva calibração linear 200-5000 ng...

& In an HPLC system the detector is the component responsible for turning a physical or chemical attribute into a measureable signal corresponding to concentration or identity. In the early days of HPLC, detection was often carried out by collecting fractions and analyzing them off-line. It wasn’t until the 1940’s and 1950’s that the first online detectors for HPLC were introduced. Certainly an...

Glutamate induces excitotoxic damage to hippocampal pyramidal neurons in Status Epilepticus (SE) and epilepsy. In this study, we investigated time course of dysregulation of calcium homeostasis at various intervals after an episode of SE in acutely isolated CA1hippocampal pyramidal. For this purpose, male Sprague-Dawley rats (200 g) were subjected to pilocarpine-induced SE. The SE was blocked a...