We are making researches into seamless integration of information distributed over networks under the SAGE (Smart AGent Environment) project. In the SAGE, conversational agents speak in ACL (Agent Communication Language) and cooperate in solving problems focused on retrieval and integration of heterogeneous information. The SAGE consists of user agents which agentify users, database agents whic...

Sagebrush (Artemisia spp.) obligate wildlife species such as the imperiled greater sage-grouse (Centrocercus urophasianus) face numerous threats including altered ecosystem processes that have led to conifer expansion into shrub-steppe. Conifer removal is accelerating despite a lack of empirical evidence on grouse population response. Using a before-after-control-impact design at the landscape ...

PURPOSE Although extraocular muscle (EOM) is skeletal muscle, aspects of its biology are unlike other striated muscle. In this study, the broad molecular genetics profile underlying the novel EOM phenotype was examined. METHODS Serial analysis of gene expression (SAGE) was used to quantify adult rat EOM gene transcripts. SAGE isolates and sequences 10-bp tags from defined locations in mRNA-de...

Serial Analysis of Gene Expression (SAGE) is a technique for estimating the gene expression profile of a biological sample. Any efficient inference in SAGE must be based upon efficient estimates of these gene expression profiles, which consist of the estimated relative abundances for each mRNA species present in the sample. The data from SAGE experiments are counts for each observed mRNA specie...

We reviewed the historic and present distribution of Greater Sage-Grouse (Centrocercus urophasianus) in Canada and found that the species has been eliminated from approximately 90% of its estimated historic distribution. Sage-grouse have been extirpated from British Columbia and reduced to remnant populations in Alberta and Saskatchewan. Estimates of the size of the population decline in Canada...

This study evaluated a relatively new molecular technique, serial analysis of gene expression (SAGE), as a tool for quantifying gene expression in the ovine abomasal nematode Haemonchus contortus for which there is relatively limited (approximately 20% gene coverage) sequence information. SAGE technology generates data that are both qualitative and quantitative and, as such, compliments other f...

Conserving migratory ungulates in increasingly human-dominated landscapes presents a difficult challenge to land managers and conservation practitioners. Nevertheless, ungulates may receive ancillary benefits from conservation actions designed to protect species of greater conservation priority where their ranges are sympatric. Greater Sage-Grouse (Centrocerus urophasianus), for example, have b...

BENCHMARKS The serial analysis of gene expression (SAGE) provides high-throughput information on researched samples. SAGE generates 9–13 bp tags from messenger RNA (mRNA) of the sample, and all tags are ligated to form concatemers for cloning and sequencing (1). LongSAGE uses similar procedures but a different restriction enzyme to obtain longer tags (19–21 bp). Both SAGE and LongSAGE tags are ...

Herbal medicines include a range of pharmacologically active compounds: in some cases it is not well understood which ingredients are important for a therapeutic effect. The supporters of herbal medicine believe that isolated ingredients in the majority of cases have weaker clinical effects than whole plant extract, a claim that would obviously require proof in each case. Generalizations about ...