نتایج جستجو برای: recombinant protein sag1

تعداد نتایج: 1297381  

Abd Emami B Abolghasemi S Ahani-Azari M Amiri Yekta A Bagheri A, Fatemi N, Gourabi H Hasani S Sanati MH

Background: Bovine follicle-stimulating hormone (bFSH) is a heterodimer hormone that consists of a common -subunit which noncovalently associated with the hormone-specific -subunit. During the past 15 years, the methylotrophic yeast Pichia pastoris has become an important host organism for recombinant protein production because it is able to use methanol as a sole carbon and energy source. Th...

تولائی, محمود , فاطمی, سید صفاعلی , مرادی, محمدطاهر , یاری, خیراله ,

Background and purpose: The 50 KDa protein (50 µg) in carboxylic domain of the neurotoxin heavy chain (BoNT/A-Hc) recognizes surface receptors on target neurons and this fragment contains the principle protective antigenic determinants. Recently, this fragment has been used as a recombinant vaccine candidate for botulism. The study aimed to compare the evaluation of BoNT/A-Hc production in fed-...

So far, recombinant antigens of HIV-1, the etiologic cause of Acquired Immunodeficiency Syndrome (AIDS), have been widely used for the diagnosis and vaccine development. P17 or the matrix protein formed by the proteolytic cleavage of gag is strongly antigenic and is as conserved and immunogenic as p24. In some cases, antibodies to p17 are more prevalent than antibodies to p24 and the decline in...

Asghar Abdoli Fatemeh Fotouhi Hoorieh Soleimanjahi, Shahram Pour Beiranvand Zahra Kianmehr

  Objective(s):  Infection by high-risk papillomavirus is regarded as the major risk factor in the development of cervical cancer. Recombinant DNA technology allows expression of the L1 major capsid protein of HPV in different expression systems, which has intrinsic capacity to self-assemble into viral-like particles (VLP). VLPS are non-infectious, highly immunogenic and can elicit neutralizing...

Asghar Tanomand, Jafar Majidi, Safar Farajnia, Shahin Najar,

Background: Infections due to Pseudomonas aeruginosa are among the leading causes of morbidity and mortality in patients who suffer from impaired immune responses and chronic diseases such as cystic fibrosis. At present, aggressive antibiotic therapy is the only choice for management of P. aeruginosa infections, but emergence of highly resistant strains necessitated the development of novel alt...

Journal: :journal of paramedical sciences 0
shabnam javanzad department of genetics, islamic azad university, tehran medical branch azam bolhassani department of hepatitis and aids, pasteur institute of iran, tehran fatemeh doustdari molecular immunology and vaccine research lab., pasteur institute of iran, tehran mehrdad hashemi department of genetics, islamic azad university, tehran medical branch abolfazl movafagh department of medical genetics, faculty of medicine, shahid beheshti university of medical sciences, tehran

the human papillomavirus l1 major capsid protein (hpv l1), the basis of the current vaccines, self-assembles into virus-like particles (vlps). herein, we describe the expression and purification of recombinant hpv16 l1 in e. coli system. the l1 protein was generated in a fused form using an inducible expression system. the recombinant gst-l1 fusion protein migrated as a 82 kda protein in sds-pa...

Introduction: Inteins (INT) are internal parts of a number of proteins in yeast and some other unicellular eukaryotes, which can be separated from the immature protein during protein splicing process. After identifying the mechanism of intein action, applications of these sequences are be considered in the single- step purification of recombinant proteins and different intein tags were develope...

Arshid Yousefi-Avarvand, Ehsan Aryan, Farzad Khademi, Kiarash Ghazvini, Mohammad Derakhshan, Mohsen Tafaghodi, Mojtaba Sankian, Zahra Meshkat,

Background: The purpose of this study was to clone, express, and purify a novel multidomain fusion protein of Micobacterium tuberculosis (Mtb) in a prokaryotic system. Methods: An hspX/esxS gene construct was synthesized and ligated into a pGH plasmid, E. coli TOP10 cells were transformed, and the vector was purified. The vector containing the construct and pET-21b (+) plasmid were digested ...

Journal: :reports of biochemistry and molecular biology 0
malihe moghadam immunology research center, medical school, mashhad university of medical sciences, mashhad, iran ali ganji immunology research center, medical school, mashhad university of medical sciences, mashhad, iran abdolreza varasteh allergy research center, medical school, mashhad university of medical sciences, mashhad, iran reza falak immunology research center, iran university of medical sciences, tehran, iran - department of immunology, iran university of medical sciences, tehran, iran mojtaba sankian tel: +98 5137112410; fax: +98 5137112596

background: recombinant proteins overexpressed in e. coli are usually deposited in inclusion bodies. cysteines in the protein contribute to this process. inter- and intra- molecular disulfide bonds in chitinase, a cysteine-rich protein, cause aggregation when the recombinant protein is overexpressed in e. coli. hence, aggregated proteins should be solubilized and allowed to refold to obtain nat...

Journal: :مجله علوم پزشکی رازی 0
زهرا ریخته گران تهرانی انستیتو پاستور ایران کیهان آزادمنش انستیتو پاستور ایران احسان مصطفوی انستیتو پاستور ایران محمد عزیزی انستیتو پاستور ایران علیرضا خبیری انستیتو پاستور ایران

background: improving the performance of hiv diagnosis assays is one of the most important ways to reduce hiv transmission. because of the high mutation rate of hiv, it is critical to use the conserved proteins to develop diagnostic immunoassay methods. because integrase is one of the most conserved proteins of hiv, it may be a good target for this purpose. in this paper cloning, purification a...

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