This article contains Supplementary Data including methods and figures that relate to the article entitled "Construction of a compatible Gateway-based co-expression vector set for expressing multiprotein complexes in E. coli" (L. Salim, C. Feger, D. Busso, 2016) [1] that describes the elaboration and the validation of a set of versatile compatible plasmids for co-expression studies in Escherich...

We studied the role of isocitrate lyase in the interaction between Mycobacterium bovis BCG and mice. ApoB100-only LDLR (B6;129S-ApoBLdlr/ J) mice were inoculated with M. bovis BCG harbouring plasmids carrying the gene for isocitrate lyase. The presence of *29 times more copies of this gene resulted in a higher bacterial yield in the spleens and lungs of the infected mice. The spleen was 3–4 tim...

Plasmids are extra-chromosomal circular double-stranded DNA molecules that can be used to develop recombinant cell lines and to deliver therapeutic genetic material. In gene therapy applications, the in-vivo insertion of the genetic vector results in the production of a desired therapeutic bio-molecule. Gene therapy is of interest in the treatment of a wide range of medical conditions including...

This study aimed to construct a novel recombinant expression vector, pcDNA3.1(-)hTERT-shRNA/yCDglyTK. Its bioactivity and antitumor effects were investigated in the SGC7901 human gastric cancer cell line. Interfering RNA (RNAi) targeting human telomerase reverse transcriptase (hTERT) was applied to construct the pYr1.1-hTERT-shRNA vector. The shRNA expression cassette (including U6 promoter) wa...

Recombinant plasmids containing mutant or wild-type adenovirus serotype 2 EIa genes that produce the 12S mRNA alone, the 13S mRNA alone, or both mRNAs were cotransfected into HeLa cells with plasmids containing the viral EIIa or EIII transcription units. The amount of RNA produced from the EIIa and EIII promoters was increased by the products of both the 13S and the 12S RNAs. By measuring the l...

sequence suggested that the enzyme belongs to the short-chain dehydrogenase!reductase family. Expression plasmids for the xith gene were constructed and used to produce recombinant strains of G. orvdans that had up to 11-fold greater XDH actiyity than the wildtype strain. When used in the pToduction of xylitol from D-arabitol under controlled aeration and pH conditions, the strain harboring the...