We report a case of scleral keratitis caused by Phomopsis phoenicicola. Pterygium surgery was a predisposing factor, and the patient was treated with natamycin and fluconazole eye drops and oral fluconazole. The fungus was identified by sequencing of the internal transcribed spacer (ITS) region of the fungal ribosomal DNA (rDNA) locus and confirmed on the basis of its typical pycnidia and conidia.

IGS is abundant in polymorphism, which widely used the analysis of intraspecific genetic diversity and phylogenetic relationships among geographical populations. In this study, 45S rDNA repeat unit Saccharina japonica was obtained for first time by BAC clone sequencing. The total length S. 8 995 bp, including 5 420 bp 18S-5.8S–25S rDNA, 3 575 (Intergenic Spacer), with GC content 51.4 %. compose...

In the present study, out of 264 phosphate (P) solubilizing Bacillus strains isolated from apple rhizosphere, only twelve isolates were found to be efficient (showed most of the plant growth promoting activity) which were further characterized at molecular level using 16S rDNA partial gene sequencing. Out of 12 isolates, MZPSB 207 was found to be most efficient P-solubilizing (864.71 μg/ml) iso...

The aim of this study was to develop appropriate protocols for flow cytometric (FCM) and 16S rDNA sequencing investigation of the microbiome in a powdered infant formula (PIF) production facility. Twenty swabs were collected from each of the three care zones of a PIF production facility and used for preparing composite samples. For FCM studies, the swabs were washed in 200 mL phosphate buffer s...

The ribosomal RNA encapsulates a wealth of evolutionary information, including genetic variation that can be used to discriminate between organisms at a wide range of taxonomic levels. For example, the prokaryotic 16S rDNA sequence is very widely used both in phylogenetic studies and as a marker in metagenomic surveys and the internal transcribed spacer region, frequently used in plant phylogen...

In the present study, six species of Bipolaris viz. B. heveae, B. neergaardii, B. papendrofii, B. zeae, B. oryzae, and B. australiensis and two species of Curvularia viz. C. intermedia and C. heteropogonicola were identified on graminicolous species. Bipolaris heveae on Echinochloa sp., Cynodon dactylon and Arundo sp., B. neergaardii on Oryza sativa, B. papendrofii on Saccharum officinarum and ...

BACKGROUND Substantial progress in high-throughput metagenomic sequencing methodologies has enabled the characterisation of bacteria from various origins (for example gut and skin). However, the recently-discovered bacterial microbiota present within animal internal tissues has remained unexplored due to technical difficulties associated with these challenging samples. RESULTS We have optimiz...

OBJECTIVES In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical str...