Desiccation tolerance of protoplasts isolated from germinating pea (Pisum sativum L. cv. 'Alaska') embryonic axes depends, in part, on the osmotic strength and composition of the suspending medium. To determine the reason for this dependence and whether treatment with different solutions results in different types of damage, protoplast recovery and survival were assessed after dehydration to a ...

Bacterial plasmolytic response to osmotic stress was evaluated as a physical indicator of membrane integrity and hence cellular viability. Digital image analysis and either low-magnification dark-field, high-magnification phase-contrast, or confocal laser microscopy, in conjunction with pulse application of a 1.5 M NaCl solution, were used as a rapid, growth-independent method for quantifying t...

• An efficient protocol for protoplasts isolation from in vitro leaf and mesocarp of oil palm has been established. Enzymes composition effect on the yield viability protoplast are significant. Scraping waxy layer increased efficiency. Vacuum treatment enhanced penetration enzymes into tissues. 10% ficoll 0.6 M mannitol improved separation impurities. The absence a cell wall contributes to its ...

The organization of actin microfilaments was studied by immunofluorescence isolated from sunflower hypocotyls and cultured in an agarose matrix. Remov wall completely disrupted the actin cytoskeleton, which became progressively into cortical microfilament arrays and actin cables during protoplast culture. protoplasts with Arg-Gly-Asp motif-containing peptides, to inhibit putative cell the agaro...

The relation between the composition of the phospholipid molecular species in a cell membrane and the velocity of protoplast fusion was studied using cells cultured at a low temperature (10 C). Cells cultured at a low temperature contained larger proportions of phospholipids of low phase transition point, the 1,2-dilinoleoyl-type, than those cultured at a normal temperature (25 C). When treated...

An assay based on light-mediated oxidation was used to determine whether specific spin labels were partitioned throughout the protoplast or retained in the plasmalemma of Avena sativa L. cv. Garry and Park. Many classes of spin label were tested, including phospholipids, fatty acid, fatty acid methyl ester, maleimide, iodoacetamide, short chain hydrocarbon, androstane, 2,2,6,6-tetramethyl-4-ami...

Cell walls are at the basis of a structural, four-dimensional framework of plant form and growth time. Recent rapid progress of cell wall research has led to the situation where the old, long-lasting juxtaposition: "living" protoplast--"dead" cell wall, had to be dropped. Various attempts of re-interpretation cast, however, some doubts over the very nature of plant cell and the status of the wa...