abstract         background and objective: hirudin is an anticoagulant polypeptide secreted from the salivary glands of leeches. recombinant hirudin is a strong anticoagulant agent in arterial and venous thrombosis. the aim of this study was to evaluate the effect of inserting protein a signal peptide sequence of pezz18 plasmid on expression and secretion of the recombinant hirudin in e.coli.  ...

abstract: cryptosporidium parvum is an apicomplexan protozoan parasite which causes diarrhea in both human and wide range of animals. since this protozoa causes remarkable economic losses in cattke industry in iran, the molecular determination of porotozoa and characterization of its protein pattern and immunogenic antigend are the aim of this study. in this study, diarheatic fecal samples of c...

Small Developing States (SDS) face substantial challenges in achieving sustainable development. Many of these relate to the small size and limited diversification their economies. SDS are also among most vulnerable countries impact climate change natural disasters. Meeting development goals goes hand-in-hand with building resilience. But additional costs meet resilience objectives difficult fin...

Though many diagnostic PCR methods have been described, few techniques have been developed to rapidly and efficiently screen transformed bacterial colonies for plasmid presence (1). Unfortunately, PCR screening of a large number of colonies is time consuming and requires substantial amounts of polymerase and primers. In this report, we describe a two-step large-scale bacterial colony screening ...

This paper describes the application of two direct and one indirect methods for the extraction of microbial community DNA from soils polluted with heavy metals. DNA was extracted directly from soil using a gentle method based on soil incubation at 37°C with proteinase K and SDS, or the method was modified by the addition of a bead beating step. The indirect approach was based on the RNA/DNA ext...

Submit Manuscript | http://medcraveonline.com Abbreviations: HR: Hypersensitive Response; SAR: Systemic Acquired Resistance; WIPK: Wound-Induced Protein Kinase; SIPK: Salicylic Acid-Induced Protein Kinase; TCA: Trichloroacetic Acid; 2-ME: 2-Mercaptoethanol; SDS: Sodium Dodecyl Sulphate; PMSF: Phenyl Methyl Sulfonyl Fluoride; 2DE: 2D Gel Electrophoresis; DTT: Dithiothreitol; aact: Acetyl-CoA C-A...

This paper characterizes the complexes formed by a small protein, ubiquitin (UBI), and a negatively charged surfactant, sodium dodecyl sulfate (SDS), using capillary electrophoresis (CE), circular dichroism (CD), and amide hydrogen-deuterium exchange (HDX; as monitored by mass spectroscopy, MS). Capillary electrophoresis of complexes of UBI and SDS, at apparent equilibrium, at concentrations of...

Background: Quantitative fluorescence-polymerase chain reaction (QF-PCR) is an inexpensive and accurate method for the prenatal diagnosis of aneuploidies that applies short tandem repeats (STRs) as a chromosome-specific marker. Despite its apparent advantages, QF-PCR is not applicable in all cases due to the presence of uninformative STRs. This study was carried out to investigate the efficienc...