نتایج جستجو برای: pcr and elisa assay

تعداد نتایج: 16875876  

2013
Boon-Teong Teoh Sing-Sin Sam Kim-Kee Tan Jefree Johari Mohammed Bashar Danlami Poh-Sim Hooi Rafi Md-Esa Sazaly AbuBakar

BACKGROUND Early and rapid detection of dengue virus (DENV) infection during the febrile period is crucial for proper patient management and prevention of disease spread. An easy to perform and highly sensitive method is needed for routine implementation especially in the resource-limited rural healthcare settings where dengue is endemic. METHODS A single-tube reverse transcription-loop-media...

2014
Yu-Ling SUN Chon-Ho YEN Ching-Fu TU

Loop-mediated isothermal amplification (LAMP) combined with enzyme-linked immunosorbent assay (LAMP-ELISA) and with lateral flow dipstick (LAMP-LFD) are rapid, sensitive and specific methods for the visual detection of clinical pathogens. In this study, LAMP-ELISA and LAMP-LFD were developed for the visual detection of canine parvovirus (CPV). For LAMP, a set of four primers (biotin-labeled for...

Journal: :دامپزشکی 0
غلامرضا نیکبخت بروجنی گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران سید مهدی امام گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران حسین رضائی گروه میکروبیولوژی دانشکده دامپزشکی دانشگاه تهران

bovine leukemia virus (blv) is a retrovirus causing a chronic leukemia/lymphoma in cattle in many countries around the world. screening for antibodies by elisa has been the primary means of detecting the presence of this virus. it is not yet known if this assay will detect antibodies in all cattle during a concomitant bovine leukemia virus infection. the polymerase chain reaction (pcr) of the t...

2006
T. Malovrh M. Pate M. Ocepek B. Krt

Bovine leukaemia virus (BLV) is a retrovirus that induces a chronic infection in cattle. Once infected, cattle remain virus carriers for life and start to show an antibody response within a few weeks after infection. Eradication and control of the disease are based on early diagnostics and segregation of the carriers. The choice of a diagnostic method depends on the eradication programme, money...

2016
Shruti Shukla Gibaek Lee Xinjie Song Jung Hyun Park Hyunjeong Cho Eun Ju Lee Myunghee Kim

This study aimed to optimize the applicability of an immunoliposome-based immunomagnetic concentration and separation assay to facilitate rapid detection of Cronobacter sakazakii in powdered infant formula (PIF). To determine the detection limit, specificity, and pre-enrichment incubation time (0, 4, 6, and 8 h), assay tests were performed with different cell numbers of C. sakazakii (2 × 100 an...

Journal: :Analytical biochemistry 2000
B U Sehgal R Dunn L Hicke H A Godwin

small standard deviations of 0.11 and 0.39 pg/ml, respectively. Additional studies demonstrated that the recovery of 0.13, 0.35, 0.62, 2.7, 24, and 260 pg/ml VEGF in 10% pooled mouse EDTA plasma was 100– 120%; the average recoveries of 2, 5, and 20 pg/ml VEGF in 10% individual human serum (n 5 4, with 98–130 pg/ml endogenous VEGF) were 83, 94, and 96%, respectively. This immuno-PCR therefore ca...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه فردوسی مشهد - دانشکده کشاورزی و منابع طبیعی 1392

انتخاب روش مناسب و سریع جهت شناسایی عوامل ویروسی که گاهاً خسارات شدیدی بر عملکرد سیب زمینی دارند از اهمیت ویژه ای برخوردار است، بدین منظور روشهای تشخیصی متعددی وجود دارد که تکنیک lamp یکی از آنها می باشد. جهت بررسی کارایی این تکنیک جهت شناسایی ویروس y سیب زمینی و مقایسه آن با روشهای pcr و elisa ابتدا تعداد 250 نمونه برگی از استان خراسان رضوی جمع آوری شد و سپس از انجام تست الایزا با هریک از نمونه...

Journal: :Tropical Journal of Pharmaceutical Research 2023

Purpose: To investigate the effect of miR-493-5p in lipopolysaccharide (LPS) -induced ATDC5 chondrogenic cells.
 Methods: The MTT assay was used to determine viability LPS-induced cells. ENCORI (starbase.sysu.edu.cn/) predict target miR-493-5p. Quantitative reverse-transcription-polymerase chain reaction (qRT-PCR) expression controlled cells and treated with various combinations LPS, negat...

Journal: :Journal of clinical microbiology 2000
L Rahalison E Vololonirina M Ratsitorahina S Chanteau

The diagnostic value of a PCR assay that amplifies a 501-bp fragment of the Yersinia pestis caf1 gene has been determined in a reference laboratory with 218 bubo aspirates collected from patients with clinically suspected plague managed in a regional hospital in Madagascar. The culture of Y. pestis and the detection of the F1 antigen (Ag) by enzyme-linked immunosorbent assay (ELISA) were used a...

Journal: :Journal of clinical microbiology 1995
H Li D T Shen D O'Toole D P Knowles J R Gorham T B Crawford

Development of control measures for the gammaherpesviral disease of cattle known as sheep-associated malignant catarrhal fever (SA-MCF) has been hampered by a lack of accurate diagnostic tests either for the causative virus or for antibody against that virus. A recently developed competitive-inhibition enzyme-linked immunosorbent assay (CI-ELISA) for the detection of antibody to malignant catar...

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