20 Staphylococcus pseudintermedius is often associated with pyoderma, which can turn into a life21 threatening disease. The dissemination of highly resistant isolates has occurred in the last 10 22 years and has challenged antimicrobial treatment of these infections considerably. We have 23 compared the carriage of virulence genes and biofilm formation between methicillin-resistant 24 and methi...

To get insight on archaeal nitrogen signaling and on haloadaptation of the nitrogen/carbon/energy-signaling protein PII, we determined crystal structures of recombinantly produced GlnK2 from the extreme halophilic archaea Haloferax mediterranei, complexed with AMP or with the PII effectors ADP or ATP, at respective resolutions of 1.49, 1.45 and 2.60 Å. A unique A cc ep te d A rt ic le This arti...

Recently, human amniotic fluid (AF) cells have attracted a great deal of attention as an alternative cell source for transplantation and tissue engineering. AF contains a variety of cell types derived from fetal tissues, of which a small percentage is believed to represent stem cell sub-population(s). In contrast to human embryonic stem (ES) cells, AF cells are not subject to extensive legal or...

Many cancer cells depend on glutamine as they use the glutaminolysis pathway to generate building blocks and energy for anabolic purposes. As a result, glutamine transporters are essential for cancer growth and are potential targets for cancer chemotherapy, with ASCT2 (SLC1A5) being investigated most intensively. Here we show that HeLa epithelial cervical cancer cells and 143B osteosarcoma cell...

The presence of a small number of infected but transcriptionally dormant cells currently thwarts a cure for the more than 35 million individuals infected with HIV. Reactivation of these latently infected cells may result in three fates 1) death due to a viral cytopathic effect, 2) death due to immune clearance, or 3) a retreat back into latency. Uncovering the dynamics of HIV gene expression an...