Standard culture-based drug susceptibility testing (DST) of Mycobacterium tuberculosis (MTB) to pyrazinamide (PZA) is difficult to perform. PZA is a prodrug that has to be converted to its active form pyrazinoic acid (POA) by pyrazinamidase (PZase) enzyme, encoded by pncA gene and loss of PZase activity is associated with PZA resistance. To further define genetic basis of PZA resistance and det...

Overexpression of pleiotropic drug resistance (PDR) efflux pumps of the ATP-binding cassette (ABC) transporter superfamily is the major cause of fungal multi-drug resistance and decreased efficacy of antifungal drugs. This review focused on recent progresses in understanding of the PDR efflux pumps of ABC transporter superfamily in Saccharomyces cerevisiae and the fungal pathogens Candida albic...

Multi Drug Resistance (MDR) in Acinetobacter baumannii is one of the major threats for emerging nosocomial infections in hospital environment. Multidrug-resistance in A. baumannii may be due to the implementation of multi-combination resistance mechanisms such as β-lactamase synthesis, Penicillin-Binding Proteins (PBPs) changes, alteration in porin proteins and in efflux pumps against various e...

OBJECTIVE Global surveillance has shown that drug resistant (DR) tuberculosis (TB) is widespread. Prompt detection of Mycobacterium tuberculosis drug resistance is essential for effective control of TB. The most frequent mutations associated with Isoniazid (INH) resistance in Mycobacterium are substitutions at codons 315 in the katG gene and the mabA-inhA promoter region (-15). This survey eval...

Medical and pharmacological communities have long searched for antimicrobial drugs that increase their effect when used in combination, an interaction known as synergism. These drug combinations, however, impose selective pressures in favour of multi-drug resistance and as a result, the benefit of synergy may be lost after only a few bacterial generations. Furthermore, there is experimental evi...

Bacterial pathogens that are multi-drug resistant compromise the effectiveness of treatment when they are the causative agents of infectious disease. These multi-drug resistance mechanisms allow bacteria to survive in the presence of clinically useful antimicrobial agents, thus reducing the efficacy of chemotherapy towards infectious disease. Importantly, active multi-drug efflux is a major mec...