Relationship between latency and period for 5-hydroxytryptamine-induced membrane responses in the Calliphora salivary gland. (1993) Fertilization and thimerosal stimulate similar calcium spiking patterns in mouse oocytes but by separate mechanisms. (19%) Phospholipase C in mouse oocytes: characterisation of ,p" and y isoforms and their possible involvement in sperm-induced Ca 2+ spiking. (1994)...

background: catsper genes are a novel family of four sperm-specific calcium channels, which indicate testis-specific expression patterns. despite the crucial role of catsper genes in the male reproduction, very little is known about the factors that regulate their expression. objective: the objective of this study was to investigate the effects of vitamin e treatment on the expression of catspe...

The extracellular zona pellucida surrounds ovulated eggs and mediates gamete recognition that is essential for mammalian fertilization. Zonae matrices contain three (mouse) or four (human) glycoproteins (ZP1-4), but which protein binds sperm remains controversial. A defining characteristic of an essential zona ligand is sterility after genetic ablation. We have established transgenic mice expre...

Past studies from this laboratory have suggested that mouse sperm binding to the egg zona pellucida is mediated by a sperm galactosyltransferase (GalTase), which recognizes and binds to terminal N-acetylglucosamine (GlcNAc) residues in the zona pellucida (Shur, B. D., and N. G. Hall, 1982, J. Cell Biol. 95:567-573; 95:574-579). We now present evidence that directly supports this mechanism for g...

Interaction between gametes during fertilization is at least in part regulated by carbohydrate moieties of the zona pellucida (ZP) and carbohydrate binding proteins of the sperm surface. This review focuses on the protein-carbohydrate interactions during the primary binding of the sperm to the ZP in different species. Synthesis, structure and composition of the ZP are summarized. The functional...

Background: Male infertility is defined as an inability to impregnate a fertile female; it is a widespread problem which is usually caused by some male factors such as low quantity and quality of sperm, specifically oligospermia and azoospermia. Objective: This study aimed to evaluate the bio-positive effects of low power density Wi-Fi radiation on the reproductive system of infertile and...

A well-characterized sperm specific protein of the Member of immunoglobulin superfamily, IZUMO1, has crucial role in fertilization by mediating sperm binding to the egg plasma membrane in the mouse. However little is known about IZUMO1 in bovine. Here, we describe the molecular cloning and expression analysis of bovine IZUMO1 (bIZUMO1). RT-PCR and Western blot analysis of the bovine tissues ind...

Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30-50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols % vs 2.40-3.11 mols %). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of methylation of the eight CpGs of the consensus sequence in sperm satellite DNA ...