Resident peritoneal macrophage from BALB/c mice were infected in vitro with Rickettsia tsutsugamushi strain Gilliam, and rickettsial growth was estimated by microscopic examination of Giemsa-stained cells. Both number of infected macrophage per culture and number of intracellular rickettsiae per cell increased with time during culture. Treatment of rickettsiae with immune serum before infection...

Using a serum-free culture system, we examined murine macrophage colony formation from bone marrow cells cultured in the presence of purified CSF-1, interleukin 3 (IL 3) or a combination of the two factors. CSF-1 supported macrophage and neutrophil-macrophage colony formation, whereas IL-3 supported the formation of various types of single lineage and multilineage colonies. CSF-1 supported more...

Hypoxia-inducible factor-1 (HIF-1), a heterodimeric transcription factor composed of the α and β subunits, regulates cellular adaptive responses to hypoxia. Macrophages, which are derived from monocytes, function as antigen-presenting cells that activate various immune responses. HIF-1α response, viability, migration, phenotypic plasticity, metabolism macrophages. Specifically, macrophage-deriv...

Probiotics play an important role against infectious pathogens, such as Escherichia coli (E. coli), mainly through the production of antimicrobial compounds and their immunomodulatory effect. This protection can be detected both on live probiotic microorganisms in inactive forms (paraprobiotics). may affect different cells involved immunity, macrophages. Macrophages are activated contact with o...

We have investigated the role of the purinergic P2X7 receptor in the formation of multinucleated giant cells in human monocyte/macrophage cultures stimulated with either concanavalin A or phytohemagglutinin. Macrophage fusion can be blocked by a P2X7-selective pharmacological antagonist or by a mAb directed against the extracellular P2X7 domain. Furthermore, macrophage cell clones expressing hi...

The spontaneous binding of murine thymocytes to macrophage monolayers was employed to separate thymocytes into macrophage-unbound and -bound subsets, and the functional reactivities of these two subpopulations were examined. Macrophage-unbound thymocytes were found to be enriched in functional subsets reactive to semi-allogeneic and allogeneic stimulating spleen cells by proliferation in mixed ...

Abstract This study evaluated the cytotoxicity of Sealapex Xpress and Real Seal XT their effect on macrophage activation. J774.1 macrophages were incubated with (0.1, 1.0, 10 mg/mL) for 24 48 h. Cell viability was assessed by MTT assay activation measured pro- anti-inflammatory cytokine production using ELISA. Data analyzed one-way ANOVA Tukey’s post-test (a=0.05). not affected 0.1 or 1.0 mg/mL...

Tumor-associated macrophages (TAMs) have been involved in growth and metastases of human canine mammary tumors. However, the prognostic importance TAM specific location tumors (CMT) was not evaluated. In this study, we evaluated potential role TAMs histologic locations – intratumoral (iTAM) stromal (sTAM), as well total macrophage (tTAM) counts indicators CMT. Clinicopathologic data from 66 ani...

Abstract Macrophages respond to environmental cues and play a crucial role in host defense, inflammation, tissue homeostasis. Macrophage activation undergoes metabolic transcriptomic reprogramming adapt tailor an appropriate response, regulating innate immunity. Reactive oxygen species (ROS) have been shown pivotal macrophage activation. Nevertheless, the detailed molecular mechanism of how red...

Hepatocellular carcinoma (HCC) is a primary liver cancer with high mortality rate and limited treatment options. Recent research has brought attention to the significant importance of intercellular communication in progression HCC, wherein exosomes have been identified as critical agents facilitating cell-to-cell signaling. In this article, we investigate impact macrophages both sources targets...