Corynebacterium glutamicum is known for its effective excretion of amino acids under particular metabolic conditions. Concomitant activities of uptake and excretion systems would create an energy-wasting futile cycle; amino acid export systems are therefore tightly regulated. We have used a DNA microarray approach to identify genes for membrane proteins which are overexpressed under conditions ...

In the preceding publication (1) mention has been made of the syntrophism existing between the mutant strains llA16 and 2OA19 of Escherichia COG. These mutants, requiring isoleucine and isoleucine plus valine respectively, were able to grow together on a minimal agar plate, free of amino acids, which would not support the growth of either mutant alone. The nature of this syntrophism forms the s...

Anteiso-fatty acids (aFA) with odd carbon number are a class of branched-chain fatty acids (BCFA) mainly produced by bacteria. Bacterial sources are also made responsible for their occurrence in the low percent-range in lipids of ruminants (meat and milk) and fish. aFAs are chiral molecules and typically occur predominantly in form of (S)-enantiomers, and their primary precursor has been noted ...

Temperature-sensitive mutations in the isoleucyl-transfer ribonucleic acid (tRNA) synthetase of yeast, ilS(-)1-1 and ilS(-)1-2, were used to examine the role of aminoacyl-tRNA synthetase enzymes in the regulation of ribonucleic acid (RNA) synthesis and enzyme synthesis in a eucaryotic organism. At the permissive temperature, 70 to 100% of the intracellular isoleucyl-tRNA was charged in mutants ...

An experimental and theoretical investigation on the chemical reactivity of fullerene cations (Cn+, n = [36, 60]) with amino acid molecules (e.g., isoleucine, C6H13NO2) is performed. The results show that, in gas phase, can react to form fullerene/amino cluster high efficiency. formation rate constants for ion–molecule collision reactions between isoleucine are estimated under pseudo-first-orde...

A study of isoleucines in protein structures solved using X-ray crystallography revealed a series of systematic trends for the two side-chain torsion angles χ1 and χ2 dependent on the resolution, secondary structure and refinement software used. The average torsion angles for the nine rotamers were similar in high-resolution structures solved using either the REFMAC, CNS or PHENIX software. How...

The objective of this study was to determine the Ile requirement in early (d 39 to 61) and late (d 89 to 109) pregnancy using the indicator AA oxidation method. The same 7 Large White × Landrace sows in their fourth parity were used in early and late pregnancy. Each sow received 6 diets based on corn, corn starch, and sugar in both early and late pregnancy at constant feed allowances (2.5 kg/d)...

E have isolated a number of mutant strains of Salmonella typhimurium that display a marked inhibition of growth when valine is added to minimal medium. Attempts to elucidate the nature of the mutation(s) in these valinesensitive strains have included enzymatic and transductional analyses, as well as growth studies, on four of these strains. The results of these studies are presented and discuss...

initial reports on the mutant strains of Salmonella typhimurium that T: eui r both isoleucine and valine for growth (ilva strains) were published concomitantly by GLANVILLE and DEMEREC ( 1960) and WAGNER and BERGQUIST (1960). These studies showed that although the available ilva strains could be divided into four complementation groups ( A , B , C and D ) on the basis of abortive transduction r...

A 21-day study was conducted to determine whether isoleucine might limit the performance of piglets fed low-crude protein (CP), amino acid (AA)-supplemented diets and to investigate the potential benefits of low-CP diets on gastrointestinal health in weaned pigs. Ninety-six piglets (initial BW = 6.44 ± 0.14 kg), housed four per pen, were randomly assigned to one of four diets, resulting in six ...