In this sensitive, specific 125I radioimmunoassay for plasma progesterone, an identical glucuronide bridge is used in the immunogen and radioligand. This apparently provides a system in which the bridge is poorly recognized and thereby avoids the excessively high affinity of the tracer for the antiserum and the consequent poor sensitivity that commonly is found in other homologous-bridge situat...

Three immune sera, raised in rabbits against synthetic peptides corresponding in sequence to predetermined regions of the HLA-DQ histocompatibility antigens, were tested for their ability to recognize phenotypically distinct human lymphoblastoid cell lines. The immune sera readily recognize the immunogen. When tested on the cells, they react poorly but seem to exhibit a certain degree of specif...

The denatured catalytic polypeptide of (Na+,K+)-ATPase of goldfish brain was purified and identified as the 32P-labeled phosphoprotein. The protein served as immunogen for the preparation of rabbit antisera for immunohistochemical application t o goldfish tissue sections, using t h e peroxidase-antiperoxidase indirect method. Labeling in brain cross-sections appears primarily in fibers of the o...

Human norovirus (HuNoV) is the leading cause of acute gastroenteritis (AGE) worldwide, which highly stable and contagious, with a few virus particles being sufficient to establish infection. Although World Health Organization in 2016 stated that it should be an absolute priority develop HuNoV vaccine, unfortunately, there currently no licensed vaccine available. The major barrier development ef...

Infectious T lymphocytes were determined in thymuses and spleens of mice acutely infected with LCM virus. Such cells appeared in newborn animals, but their numbers declined when the age at infection increased and none were found in adult mice. Either susceptibility to LCM virus is associated with some degree of immaturity or the probability of murine T lymphocytes to encounter the virus as an i...

A simple method for concentrating, isolating, and purifying PF/cholera enterotoxin from culture supernatant fluids is presented. Precipitation with dextran sulfate and ammonium sulfate, followed by gel filtration and ion-exchange chromatography, yielded PF/enterotoxin of high biological potency. Although not completely free from traces of vibriocidal antibody-stimulating activity, the resultant...

This paper describes studies based on the hypothesis that the immunogenicity of the gonococcus is impaired by a component toxic to immunocytes. Cytoplasm of colony type 1 gonococci was found to contain a protein fraction beta+t not present in colony type 4 gonococci. From the results of further analysis it is tentatively deduced that beta+t consists of a toxic component Tbeta-t and an immunogen.