PURPOSE The aim of this study was to identify glucocorticoid induced cataracts (GIC)-specific modified water insoluble-urea soluble (WI-US) crystallins and related changes after rat lens were exposed to dexamethasone (Dex). METHODS We separated WI-US lens proteins by two-dimensional electrophoresis (2-DE). The crystallins were then analyzed with matrix assisted laser desorption/ionization tim...

BACKGROUND Systemic sclerosis (SSc) is an autoimmune disease characterized by immunological and vascular abnormalities. Until now, the cause of SSc remains unclear. Sclerodermatous graft-versus-host disease (ScGVHD) is one of the most severe complications following bone marrow transplantation (BMT) for haematological disorders. Since the first cases, the similarity of ScGVHD to SSc has been rep...

Arsenic (As) is broadly distributed due to natural and anthropogenic sources, it toxic organisms. This study aimed investigate the proteomic response in earthworm exposed As3+. Earthworms were As3+ soil at 5–80 mg kg?1, samples collected after 60 days exposure. Two-dimensional electrophoresis (2-DE) was used separate proteins homogenate, then differentially expressed (DEPs) identified using MAL...

AIM The present study was undertaken to clone, express and study the immunogenicity of P67 protein of Mycoplasma leachii. MATERIALS AND METHODS P67 gene was amplified from genomic DNA of M. leachii. The polymerase chain reaction (PCR) product was inserted in pRham N-His SUMO Kan vector and was used to transform competent Escherichia cloni 10G cells. Recombinant protein expression was done by ...

BACKGROUND Rapeseed-mustard is the second most important source of edible oil in India. Several species of Brassica are grown in different parts of country for its oilseeds. OBJECTIVE The objective was to investigate allergenicity to antigenic extracts of pollen of 4 species of Brassica. METHODS Brassica campestris, Brassica juncea, Brassica nigra, and Brassica napus were selected for the d...

We measured physiological functionalities, including antihypertensive angiotensin I-converting enzyme inhibitory activity and immun-stimulating β-glucan content for sixty kinds of Makgeolli that is commercially available from the market. As a result, we selected R-12 commercial raw Makgeolli, with a high content of immuno-stimulating β-glucan, and R-14 commercial raw Makgeolli, exhibiting high ...

An outbreak of goatpox was attended in district Durg and surrounding regions of Chhattisgarh state. Dried skin scabs were collected from 250 goats of different age groups showing clinical signs suggestive of pox. The prevalence rate was studied based on severity of clinical signs followed by confirmation with agar gel immuno diffusion (AGID) test. Positive scabs were further processed for virus...

Protein N-glycosylation patterns are known to show vast genetic as well as physiological and pathological variation and represent a large pool of potential biomarkers. Large-scale studies are needed for the identification and validation of biomarkers, and the analytical techniques required have recently been developed. Such methods have up to now mainly been applied to complex mixtures of glyco...

A novel approach for the analysis of membrane proteins involved in ligand-induced surface receptor patching and capping is described. The technique is based on the use of immunolactoperoxidase (immuno-LPO) conjugates which catalyze the iodination of those surface proteins with available tyrosine groups that are located in the immediate vicinity of the patch or cap of a particular antigen. We ha...

background and aims: hiv is spreading rapidly among people word wide. infection with this virus leads to immune suppression and finally acquired immune deficiency syndrome (aids).early hiv detection is depended on antibody screening against virus by enzyme linked immunosorbent assay (elisa).some confirmatory tests such as western blot and recombinant immunobloting assay (riba), are used to veri...