BACKGROUND Indisputable population exposure to widespread electromagnetic fields, has grown concerns over the probable health effects of these fields. OBJECTIVE The present study was aimed to examine the possible effects of 50 Hz extremely low frequency electromagnetic field (ELF-EMF) exposure on the number and quality of mice blastocysts. MATERIALS AND METHODS In present study, 66 NMRI pre...

We investigated the effects of exposure time and concentration of trichostatin A (TSA) on in vitro development and quality of bovine SCNT embryos. At multiple time points, the relative expression of genes related to pluripotency and reprogramming was analyzed in order to assess the quality of preimplantation embryos cultured in media with TSA using real-time PCR. Development into blastocysts wa...

The reaggregation and subsequent development of a range of disaggregated embryos has been examined: 1. Following complete dissociation embryos from 8-cell to late blastocyst reaggregated and developed to form morphologically normal blastocysts, even when blastomeres of two different developmental stages were present in the reaggregate. 2. Dissociated mid-blastocysts could also reaggregate to fo...

STUDY QUESTION What is the effect of artificial shrinkage by laser-induced collapse before vitrification on the implantation potential after transfer of vitrified-warmed blastocysts? SUMMARY ANSWER The artificial shrinkage by laser-induced collapse did not significantly increase the implantation rate per transferred collapsed blastocyst (37.6%) compared with non-collapsed blastocysts (28.9%) ...

Research Question: Is maternal age only a gross predictor of chromosome abnormalities in human embryos? Design: Here, we evaluated the less-studied variation abnormality rates embryos patients within same group. Patients undergoing IVF and PGD for chromosomal ~127 different clinics were included. PGT-A analysis was performed by single reference laboratory using array CGH or NGS. To get an estim...