Abstract Current DNA base editors contain nuclease and deaminase that enables deamination of cytosine (C) or adenine (A), but no method for guanine (G) thymine (T) editing is available now. Here we developed a deaminase-free glycosylase-based editor (gGBE) with G ability, by fusing Cas9 nickase engineered N-methylpurine glycosylase protein (MPG). By several rounds MPG mutagenesis via unbiased r...

Background: The role of androgens in acne pathophysiology has been supported by long-standing clinical and experimental observations. Methods: Genotyping for the androgen receptor gene was performed PCR-based fragment length analysis, aiming to investigate cytosine-adenine-guanine (CAG) repeat polymorphism Egyptian nodulocystic patients clarify its relevance pathogenesis. Detailed frequency CAG...

Nitrogen mustard anticancer drugs generate highly reactive aziridinium ions that alkylate DNA. Monoadducts arising from reaction with position N7 of guanine residues are the major DNA adducts generated by these agents. Interstrand cross-links in which the drug bridges position N7 of two guanine residues are formed in low yields relative to those of the monoadducts but are generally thought to b...

We revisit the topic of human genome guanine-cytosine (G+C) content and adenine-thymine (A+T) under neutral evolution. For this study, “gold standard” de novo mutation data within is used to estimate rates, instead using base substitution between related species. define rates (coefficients) events, which are estimated solely from event data. Based on mutations collected intergenic regions contr...

DNA codons, consisting of triplet nucleotides (NTs), could play important roles for RNA transcription and protein translation in living systems. Therefore, their recognition be seen diagnosis therapy purposes. Based on sequence formations Adenine (A), Guanine (G), Cytosine (C) Thymine (T) NTs, 64 codons were investigated this work regarding complexation with a molecular cubane (CUB) wire. To ac...

A hypothesis of the evolution of the genetic code is proposed, the leading mechanism of which is the nucleotide spontaneous damage leading to AT-enrichment of the genome. The hypothesis accounts for stability of the genetic code towards point mutations, the presence of code dialects, and the symmetry of the genetic code table. To account for the extant structure of the genetic code (all dialect...

a:4:{s:10:"Background";s:205:"In this study, we aimed to identify the association of SNPs candidate genes of TNF-α and IL-6 with hormones levels and sperm cells death in infertile subjects of Uttar Pradesh population in North India";s:19:"MaterialsAndMethods";s:704:"The study population comprised fertile donor (control group) and infertile group patients i.e. normozoospermic (idiopathic unexpla...

DNA bending induced by six DNA (cytosine-5) methyltransferases was studied using circular permutation gel mobility shift assay. The following bend angles were obtained: M.BSP:RI (GG(m5)CC), 46-50 degrees; M.HAE:III (GG(m5)CC), 40-43 degrees; M.SIN:I (GGW(m5)CC), 34-37 degrees; M.SAU:96I (GGN(m5)CC), 52-57 degrees; M.HPA:II (C(m5)CGG), 30 degrees; and M.HHA:I (G(m5)CGC), 13 degrees. M. HAE:III w...