Proton magnetic resonance was used to study the interactions between nucleosides and phosphate monoanion in dimethyl sulfoxide. Ribose was able to form two mutually exclusive 1:1 complexes involving either OH3' and OH5' or OH3' and OH2' as hydrogen bond donor groups. Deoxyribose could form only one of these complexes. A specific interaction of phosphate with the base moiety of nucleosides was o...

The voltammetric behaviour of the purine base guanine and the corresponding n,.,c!eoside guanosine at a glaxgy carbon ek~rode was investigated with the help of ultrasound. The adsorption of guanine and guanosiae as well as the adsorption of tJieir ox~Kion pf~:gh~ affects the overall voltammetric characteristics dramatically. In particular, the effect of ultrasound on the simultaneous adsoq~aa o...

Nucleic acids can be sequenced by a chemical procedure that partially damages the nucleotide positions at their base repetition. Many methods have been reported for the selective recognition of guanine. The accurate identification of guanine in both single and double regions of DNA and RNA remains a challenging task. Herein, we present a new, non-toxic and simple method for the selective recogn...

In many prokaryotes, asymmetrical mutational or selective pressures have caused compositional skews between complementary strands of replication arms, especially sensitive in the distribution of guanine and cytosine. In Escherichia coli, most of the guanine/cytosine skew is caused by mutation rates differing on leading and lagging strands, but contribution of skewed functionally important guani...

The influence of the gate voltage or base pair ratio modulation on λ-DNA FET performance was examined. result indicated that captured electrons in guanine molecules greatly influenced Id-Vd characteristics, and tendency conductivity partly clarified by considering activation energy holes length numbers serial AT GC sequences over which jumped. In addition, dimensionality DNA molecule discussed ...

Two methods of preparing guanine in the gas phase, thermal vaporization and laser desorption, have been investigated. The guanine generated by each method is entrained in a molecular beam, single-photon ionized with tunable VUV synchrotron radiation, and analyzed using reflectron mass spectrometry. The recorded photoionization efficiency (PIE) curves show a dramatic difference for experiments p...

Certain proAB deletion mutants of Salmonella typhimurium were found to be simultaneously deleted in a gene required for the utilization of guanine and xanthine (designated gxu). These mutants were resistant to 8-azaguanine and when carrying an additional pur mutation were unable to use guanine or xanthine as a purine source. The defect was correlated with deficiencies in the uptake and phosphor...