The occurrence of the novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), responsible for coronavirus disease 2019 (COVD-19), represents a catastrophic threat to global health. Protruding from viral surface is densely glycosylated spike (S) protein, which engages angiotensin-converting enzyme 2 (ACE2) mediate host cell entry. However, studies have reported susceptibility in intr...

EDEM1 is a mannosidase-like protein that recruits misfolded glycoproteins from the calnexin/calreticulin folding cycle to downstream endoplasmic reticulum associated degradation (ERAD) pathway. Here, we investigate the role of EDEM1 in the processing of tyrosinase, a tumour antigen overexpressed in melanoma cells. First, we analyzed and modeled EDEM1 major domains. The homology model raised on ...

Bacillus anthracis has long been considered a potential biological warfare agent, and therefore, there is a need for a safe, low-cost and highly efficient anthrax vaccine with demonstrated long-term stability for mass vaccination in case of an emergency. Many efforts have been made towards developing an anthrax vaccine based on recombinant protective antigen (rPA) of B. anthracis, a key compone...

The melanocortin-2 (MC2) receptor accessory protein (MRAP) is required for trafficking of the G protein-coupled MC2 receptor to the plasma membrane. The mechanism of action and structure of MRAP, which has a single transmembrane domain, are unknown. Here, we show that MRAP displays a previously uncharacterized topology. Epitopes on both the N- and C-terminal ends of MRAP were localized on the e...

Background Recent studies suggested Contactin-associated protein-like 2 (CNTNAP2) as a novel risk gene for Alzheimer’s disease (AD). Reduced expression levels of CNTNAP2 were found in the brains AD patients. However, mechanisms underlying protein degradation remain elusive. Method HEK and N2a cells transfected with human plasmid treated cycloheximide, lysosome inhibitors, proteasome inhibitors....

illiteracy in studies on quality of life (qol) and self-efficacy (se) of diabetic patients has been ignored. illiteracy and diabetes have high rates in developing countries. the aim of this study was to identify the effect of literacy level on health outcomes. to achieve this, we studied health outcomes such as se, self-management behaviors and health-related quality of life (hrqol) in diabetic...

Glycogen is present in all tissues, but it primarily stored the liver and muscle. As a branched chain carbohydrate, broken down by phosphorylase debrancher enzymes, which are cytoplasmic. It also degraded lysosomal α-glucosidase (GAA) known as acid α-glucosidase. The deficiency of GAA patients Pompe disease, phenotypes infantile, juvenile later onset forms. disease treated enzyme replacement th...

BACKGROUND T cell immunoglobulin and mucin domain containing 3 protein (Tim-3) expressed on terminally differentiated Th1 cells plays a suppressive role in Th1-mediated immune responses. Recently, it has been shown that N-glycosylation affects the binding activity of the Tim-3-Ig fusion protein to its ligand, galectin-9, but the binding properties of non-glycosylated Tim-3 on CD4(+)CD25(+) T ce...

Objective: This study aims to explore the value of comprehensive intervention in clinical treatment patients with type 2 diabetes mellitus complicated hypertension. Methods: 90 and hypertension were selected divided into a control group an according random number table method, 45 cases each group. The received conventional treatment, on basis group, including diet plan, exercise program, scient...