The complete amino acid sequence of rabbit skeletal muscle glycogen synthase was deduced from cDNA clones with a composite length of 3317 bp. An mRNA of 3.6 kb was identified by Northern blot analysis of rabbit skeletal muscle RNA. The mRNA coded for a protein of 734 residues with a molecular weight of 83,480. The deduced NH2-terminal and COOH-terminal sequences corresponded to those reported f...

The question as to whether or not the liver is able to form glycogen from lactic acid is of importance for the relation between the carbohydrate metabolism of the muscles and that of the liver. It is known that the process glycogen * lactic acid occurs in the muscles, while the process glycogen e glucose occurs in the liver. Glucose derived from liver glycogen is convertible into muscle glycoge...

The question as to whether or not the liver is able to form glycogen from lactic acid is of importance for the relation between the carbohydrate metabolism of the muscles and that of the liver. It is known that the process glycogen * lactic acid occurs in the muscles, while the process glycogen e glucose occurs in the liver. Glucose derived from liver glycogen is convertible into muscle glycoge...

Chemical studies on the metabolism of glucose, fructose and glycogen in human chorionic villi are reported with the following conclusions: 1) In chorionic villi the level of glucose was the mean value between that of the maternal blood and the fetal blood. The level of fructose was three times as high as that in the fetal blood. 2) Chorionic villi convert glucose into fructose. The best conditi...

Antihyperglycemic potential of hyperin at 25 and 50 mg/kg doses for 30 days to streptozotocin induced diabetic rats has been reported. In oral glucose tolerance test, hyperin treated rats showed a significant reduction in blood glucose level after 120 min. It was found that hyperin exhibited dose dependent and significant antihyperglycemic activity in streptozotocin induced diabetic rats which ...

Sarcoplasmic vesicles and ~-glycogen particles 30-40 m# in diameter were isolated from perfused rabbit skeletal muscle by the differential precipitation-centrifugation method. This microsomal fraction was subjected to zonal centrifugation on buffered sucrose gradients, in a B X I V Anderson type rotor, for 15 hr at 45,000 rpm in order to separate the two cytoplasmic organelles. Zonal profiles o...

BACKGROUND Type 2 diabetes afflicts 95% of diabetes patients. Recent data suggest that miRNAs play a key role in insulin production, secretion and function. This study aims to explore the specific role of miR-499-5p in hepatic insulin resistance. METHODS The miRNA expression levels in the livers of db/db mice were analyzed using miRNA chips and were verified by real-time PCR. miR-499-5p mimic...

In recent years there have been many studies concerning the metabolic disturbances in shock secondary to injury, hemorrhage, and burns. Among the very marked changes reported are t,hose involving carbohydrates. The glycogen stores of liver (1) and muscle are reduced in rats shocked by limb injury, and these tissues are unable to replenish their glycogen stores after glucose administration. The ...

This study compared the effects of coingesting glucose and fructose on exogenous and endogenous substrate oxidation during prolonged exercise at altitude and sea level, in men. Seven male British military personnel completed two bouts of cycling at the same relative workload (55% Wmax) for 120 min on acute exposure to altitude (3375 m) and at sea level (~113 m). In each trial, participants inge...

objective(s):the application of stem cells holds great promises in cell transplants. considering the lack of optimal in vitro model for hepatogenic differentiation, this study was designed to examine the effects of laminin matrix on the improvement of in vitro differentiation of human bone marrow mesenchymal stem cells (hbm-msc) into the more functional hepatocyte-like cells. materials and meth...