A simple reproducible in vitro B-cell colony assay system was used to evaluate B-cell growth in controls and patients with chronic lymphocytic leukemia (CLL). All six CLL patients studied formed B-cell colonies. The number of colonies was significantly less in patients than controls (66 +/- 18 versus 127 +/- 8). CLL colonies were shown to be monoclonal and appeared to reflect the circulating ma...

Figure 1. May-Grünwald-Giemsa staining (original magnification, ϫ1000) of the patient's bone marrow aspirate (left panel), showing unstained oblong shapes (white arrows) in the cytoplasma of several macrophages, and Ziehl-Neelsen staining of the same area (right panel), revealing the unstained objects to be acid-fast bacilli (black arrows). Diagnosis: hemophagocytic syndrome due to disseminated...

The data augmentation method is known as a helpful technique to generate dataset with large number of images from one small for supervised training in deep learning. However, low validity image recognition was reported recent study on artificial intelligence (AI). This aimed clarify the optimal learning model generation white blood cells (WBCs). Study Design: We conducted three different method...

The aim of this study was to evaluate different methods for Trichomonas vaginalis diagnosis during pregnancy in order to prevent maternal and perinatal complications. A total of 386 vaginal exudates from pregnant women were analyzed. T. vaginalis was investigated by 3 types of microscopic examinations direct wet mount with physiologic saline solution, prolonged May-Grunwald Giemsa (MGG) stainin...

AIM Tropical theileriosis is fatal hemoprotozoal disease of dairy animals caused by Theileria annulata. The aim of the present study was to detect the T. annulata and comparison of results of molecular and microscopic techniques. MATERIALS AND METHODS A total of 52 blood samples were collected from the cattle suspected for theileriosis across the Banaskantha district. All the samples were scr...

The stains commonly used for staining blood films (Giemsa's, Wright's, Leishman's, etc.) have become expensive, and in many cases difficult to obtain. In order to get the best results with these stains, acetone-free alcohol and distilled water of a specified pH are necessary. This again increases costs and complicates technique. Furthermore, films, and particularly thick-drop slides, are usuall...

Retrospective evaluation of potassium hydroxide plus calcofluor white (KOH+CFW), Gram, Giemsa, and modified Ziehl-Neelsen (1% H(2)SO(4), cold) stains for the detection of microsporidia in corneal scrapings from 30 patients showed KOH+CFW and acid-fast stains to be most efficient (29/30 [96.7%] and 28/30 [93.3%], respectively) in the diagnosis of microsporidial keratitis.

INTRODUCTION The present study was carried out from March to May 2008, with the aim of evaluating the effectiveness of different techniques for diagnosing Blastocystis hominis in a sample of the population attended at the Biomedicine Laboratory of Feevale University, Novo Hamburgo, Rio Grande do Sul. METHODS On hundred feces samples from children and adults were evaluated. After collection, t...

Objective. To assess the vaginal inflammatory status (VIS) in pregnant women, whether symptomatic or asymptomatic, by leukocyte quantification in relation to the microbiota during each pregnancy trimester (T). Materials and Methods. A thousand two hundred and forty eight vaginal exudates from pregnant women were prospectively examined. All the patients underwent a clinical and colposcopic exami...

objectives: the study of erythropoiesis needs to develop the methods for erythroid progenitor cells (epcs) culture using stem cell potency to differentiate into variety of hematopoietic cells lineages. in this study, we induced differentiation of cord blood stem cells into erythroid progenitor cells in a semisolid culture media. materials and methods: cord blood mononuclear cells were isolate...