To study rhodopsin biosynthesis and transport in vivo, we engineered a fusion protein (rho-GFP) of bovine rhodopsin (rho) and green fluorescent protein (GFP). rho-GFP expressed in COS-1 cells bound 11-cis retinal, generating a pigment with spectral properties of rhodopsin (A(max) at 500 nm) and GFP (A(max) at 488 nm). rho-GFP activated transducin at 50% of the wild-type activity, whereas phosph...

Leukocyte trafficking to the central nervous system (CNS), regulated in part by chemokines, determines severity of the demyelinating diseases multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). To examine chemokine receptor CX3CR1 in EAE, we studied CX3CR1(GFP/GFP) mice, in which CX3CR1 targeting by insertion of Green Fluorescent Protein (GFP) allowed tracking of CX3CR1...

Abstract Recent studies have demonstrated potent oncolytic effects of wild-type Zika virus (ZIKV) against primary central nervous system (CNS) tumors, including Medulloblastoma, Atypical Teratoid/Rhabdoid Tumor (AT/RT), and Glioblastoma (GBM). However, the neurotropism ZIKV urges further evaluation specific tumor-targeting properties comparative toxicity to non-neoplastic neural cells in order ...

We have formulated a numerical model that simulates the accumulation of green fluorescent protein (GFP) in bacterial cells from a generic promoter-gfp fusion. The model takes into account the activity of the promoter, the time it takes GFP to mature into its fluorescent form, the susceptibility of GFP to proteolytic degradation, and the growth rate of the bacteria. From the model, we derived a ...

To investigate the effects of the centrosomal protein, ninein-like protein (Nlp), on the proliferation, invasion and metastasis of MCF-7 breast cancer cells, the present study established green fluorescent protein (GFP)-containing MCF7 plasmids with steady and overexpression of Nlp (MCG7-GFP-N1p) and blank plasmids (MCF7-GFP) using lentiviral transfection technology in MCF7 the breast cancer ce...

background: oncolytic herpes simplex virus (ohsv) vectors lacking γ34.5 has demonstrated anticancer effects without affecting normal cells. therefore, they are considered as ideal templates to construct efficient vectors for tumor targeting and cancer gene therapy. herein, we reported construction of three single/dually-labeled recombinant hsv-1 vectors, which provide an opportunity to replace ...

•UBQLN2 is recruited to reduce poly-GA aggregation and alleviate its neurotoxicity•UBQLN2 can recognize HSP70 ubiquitination facilitate degradation•The UBQLN2-HSP70 interaction required for degradation•Pharmacologically enhancing mitigates behavioral defects in mice Expansion of a hexanucleotide repeat GGGGCC (G4C2) the intron C9ORF72 gene most common cause amyotrophic lateral sclerosis (ALS) f...