نتایج جستجو برای: fungal dna

تعداد نتایج: 556901  

2016
S. Umesha H. M. Manukumar Sri Raghava

Food contaminated with fungal pathogens can cause extremely harmful effects to human even when present in low concentrations. Researchers now pay more attention towards rapid DNA extraction for the quick screening, which is highly demanded in diverse research field. Molecular description of many fungal species is identified by different molecular characteristics. Hence, the efficient isolation ...

2014
Dan Norbäck Pawel Markowicz Gui-Hong Cai Zailina Hashim Faridah Ali Yi-Wu Zheng Xu-Xin Lai Michael Dho Spangfort Lennart Larsson Jamal Hisham Hashim

There are few studies on associations between respiratory health and allergens, fungal and bacterial compounds in schools in tropical countries. The aim was to study associations between respiratory symptoms in pupils and ethnicity, chemical microbial markers, allergens and fungal DNA in settled dust in schools in Malaysia. Totally 462 pupils (96%) from 8 randomly selected secondary schools in ...

Journal: :Food microbiology 2008
Jean-Luc Jany Georges Barbier

This review focuses on the culture-independent methods available for the description of both bacterial and fungal communities in cheese. Important steps of the culture-independent strategy, which relies on bulk DNA extraction from cheese and polymerase chain reaction (PCR) amplification of selected sequences, are discussed. We critically evaluate the identification techniques already used for m...

Journal: :Japanese journal of ophthalmology 1998
K Mochizuki H Matsushita Y Hiramatsu K Yanagida G Ueda K Hashimoto

We evaluated the vitreous bodies from 14 clinically nonfungal infectious eyes to establish a baseline for the presence of a fungal genome using polymerase chain reaction (PCR). The vitreous samples were all PCR negative, except for one eye with chronic uveitis. Blood samples examined were all PCR negative. We concluded that fungal DNA sequences could not be amplified in vitreous specimens obtai...

2012
Altamiro Ribeiro Dias Filomena Marino Carvalho Heloisa de Andrade Carvalho Maria Del Pilar Esteves Diz Edmund Chada Baracat Jesus Paula Carvalho

Fanconi Anemia (FA) is an autosomal recessive disease characterized by chromosome instability, cellular hypersensitivity to DNA cross-linking agents, and increased predisposition to malignancies. We describe here a 28 year-old female with FA and vaginal squamous cell carcinoma treated by radiation therapy alone. The patient developed arm phlebitis, pulmonary fungal infection, and severe rectal ...

2012
Ekachai Chukeatirote Sajeewa S. N. Maharachchikumbura Shannaphimon Wongkham Phongeun Sysouphanthong Rungtiwa Phookamsak Kevin D. Hyde

Genes encoding the cellobiohydrolase enzyme (CBHI), designated as cbhI, were isolated from the basidiomycetes Auricularia fuscosuccinea, Pleurotus giganteus, P. eryngii, P. ostreatus, and P. sajor-caju. Initially, the fungal genomic DNA was extracted using a modified cetyltrimethyl ammonium bromide (CTAB) protocol and used as a DNA template. The cbhI genes were then amplified and cloned using t...

اجلی, محسن, بدلی, حمید, حمزه‌یی, حسین, رهنما, مهدی,

Background and Objective: Epidemiological studies indicate that not only the incidence of fungal infections is dramatically on the rise, especially in the immunocompromised hosts, but also the sensitivity of etiological agents to antifungal drugs shows a remarkable reduction. Therefore, early detection at the species level is critically important for proper clinical management. Because standard...

Journal: :The Biochemical journal 1997
B A Keller S Patel L M Fisher

Candida albicans topoisomerase II, encoded by the TOP2 gene, mediates chromosome segregation by a double-strand DNA break mechanism and is a potential target for anti-fungal therapy. In this paper, we report the characterization of the C. albicans TOP2 gene and its use to develop a yeast system that allows the identification and study of anti-fungal topoisomerase II inhibitors in vivo. The gene...

Journal: :Canadian journal of microbiology 2006
Nabla Kennedy Eoin Brodie John Connolly Nicholas Clipson

Seasonal and management influences on the fungal community structure of two upland grassland soils were investigated. An upland site containing both unimproved floristically diverse (U4a) and improved mesotrophic (MG7b) grassland types was selected. Samples from both grassland types were taken at five times in one year. Soil fungal community structure was assessed using fungal automated ribosom...

2012
Altamiro Ribeiro Dias Marcela Cavalcante de Andrade Silva Filomena Marino Carvalho Heloisa de Andrade Carvalho Maria Del Pilar Esteves Diz Edmund Chada Baracat Jesus Paula Carvalho

Fanconi Anemia (FA) is an autosomal recessive disease characterized by chromosome instability, cellular hypersensitivity to DNA cross-linking agents, and increased predisposition to malignancies. We describe here a 28 year-old female with FA and vaginal squamous cell carcinoma treated by radiation therapy alone. The patient developed arm phlebitis, pulmonary fungal infection, and severe rectal ...

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