We are pleased that de Velasco et al. [1] have compared the performance of a 34-gene model predictor (ClearCode34) developed by several authors [2] with our 8-gene predictor [3] in an independent RNA-sequencing dataset derived from frozen metastatic renal cell carcinoma (mRCC) samples [4]. We are also glad that that the authors have successfully reproduced our analyses as reported in reference ...

cause and that this, in mammals as in birds, is a virus.― Mann (33—35)injected frozen spontaneous and transplanted mammary cancers and explained the development of tumors on the basis that the mam mary tumor milk agent-virus was liberated in its active form following freezing, since mammary tu mors arose at the site of injection of the suspended frozen tumor-mince. The tumors developed as r...

SUMMER 2008 BETA 31 Trauma : “I can’t remember anything after the first few words she said: ‘I’m sorry to tell you that your test results came back positive.’ After that, it was all just a blur of sounds and images—the traffic outside, my heart beating, the look on her face. Everything was moving in slow motion. I just kept hearing those five words echoing in my head: ‘Your test came back posit...

This unit provides a robust, reliable, and easy-to-use kit-based method for extraction of intact, non-degraded proteins from formalin-fixed, paraffin-embedded (FFPE) tissue, and their subsequent use for analysis by liquid chromatography/mass spectrometry (LC/MS). After deparaffinization, proteins are extracted from unstained sections of FFPE rat liver tissue. After a simple cleanup step using o...

Survival in the frozen state depends on biochemical adaptations that deal with multiple stresses on cells including long-term ischaemia and tissue dehydration. We investigated whether the AMP-activated protein kinase (AMPK) could play a regulatory role in the metabolic re-sculpting that occurs during freezing. AMPK activity and the phosphorylation state of translation factors were measured in l...

BACKGROUND Ovarian tissue banking is an emerging strategy for fertility preservation which has led to several viable pregnancies after transplantation. However, the standard method of slow cooling was never rigorously optimized for human tissue nor has the extent and location of ice crystals in tissue been investigated. To address this, we used cryo-scanning electron microscopy (cryo-SEM) to st...

BACKGROUND The objective of this study was to investigate serial changes for histology of joint capsule and range of motion of the glenohumeral joint after immobilization in rats. We hypothesized that a rat shoulder contracture model using immobilization would be capable of producing effects on the glenohumeral joint similar to those seen in patients with frozen shoulder. METHODS Sixty-four S...

BACKGROUND Abnormal connectivity across brain regions underlies many neurological disorders including multiple sclerosis, schizophrenia and autism, possibly due to atypical axonal organization within white matter. Attempts at investigating axonal organization on post-mortem human brains have been hindered by the availability of high-quality, morphologically preserved tissue, particularly for ne...