The present work deals with the development of solid state fermenter for enzymes production and the development of a pilot scale of enzymatic deinking system. The newly developed solid state fermenter, was named FERMSOSTAT®, which was used for the production of cellulases and xylanase using a local isolate; Aspergillus niger USM AI 1. Optimization of enzymes production was carried out and the o...

l-asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.in this study, simple and advantageous method has been validated for rapid and precise determination of intracellular l-asparaginasein bacterial species. a suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...

Konjac oligo-glucomannan (KOG) was prepared by degradation of konjac glucomannan (KG) using β-mannanase. The hydrolysis process was monitored by the viscosity of the enzymatic hydrolysates. Factors affecting the enzymatic hydrolysis of KG were investigated, and the optimum hydrolysis conditions were as follows: time 2h; temperature 50°C; pH 6.0; and enzymatic concentration 150 U/g. Under these ...

BACKGROUND Patients suffering from chronic kidney disease (CKD) are exposed to increased oxidative stress and disturbances manifesting in the enzymatic and non-enzymatic antioxidative defence system. The object of the research was to assess the differences between conservative treatment, peritoneal dialysis and haemodialysis in moderating cellular antioxidative agents. METHODS The group exami...

This study investigated the degradation and enzymatic saccharification of cornstalk by white-rot and brown rot fungi. The fungal strains Trametes pubescens Cui 7571, Trametes velutina Dai 10149, and Antrodia wangii Cui 7568 were analyzed in solid-state fermentation cultures. Various extracellular enzyme activities were assessed to determine biochemical changes during the degradation process. Fo...

The Ten-eleven translocation (TET) proteins have been recently identified as critical regulators in epigenetic modification, especially in the methylation of cytosine in DNA. TET-mediated DNA oxidation plays prominent roles in a wide variety of physiological and pathological processes, especially in tumor and neural development. TET proteins execute stepwise enzymatic conversion of 5-methylcyto...

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is the most widely studied ligand of the aryl hydrocarbon receptor (AHR). The AHR-dependent TCDD-induced mitochondrial hyperpolarization (Tappenden et al., 2011) [1] and reduced oxygen consumption rate of intact mouse hepatoma cells (Huang et al., in press) [2] in the previous studies suggest that these alterations can be related to enzymatic activitie...

Non-enzymatic glycosylation reaction, which proceeded at an accelerated rate in diabetes, directly caused sharp diminution of total haemoglobin due to glycosylated proteins including haemoglobin digested by macrophages. The diminution contributed to hypoxia of tissue that repressed the enzymatic activities in the respiratory chain as well as in the tri-carboxylic acid cycle (TCA) and Embden-Mey...

Cyclic nucleotide phosphodiesterase has been prepared from the liver, skeletal muscle, heart muscle, kidney, epididymal fat pad, and brain cortex of the rat. Kinetic analysis indicated that all of these tissues, with the exception of liver exhibit complex behavior indicative of multiple enzymatic activities. The phosphodiesterase activities of brain cortex, kidney, and adipose tissue have been ...

ERK2 are protein kinases that during the enzymatic catalysis, in contrast to traditional enzymes, utilize additional interactions with substrates outside of the active sites. It is widely believed that these docking interactions outside of the enzymatic pockets enhance the specificity of these proteins. However, the molecular mechanisms of how the docking interactions affect the catalysis remai...