This protocol describes the mounting of cells which have been grown on cleaned coverslips in cell culture dishes containing the appropriate medium and supplements. Labeling is performed with the coverslips placed in culture dishes. After cells are labeled, the coverslips can be mounted onto microscope slides to be viewed for short-term observation of live cells or longer-term observation of fix...

The nerve growth cone binds to a complex array of guidance cues in its local environment that influence cytoskeletal interactions to control the direction of subsequent axon outgrowth. How this occurs is a critical question and must certainly involve signal transduction pathways. The paper by Suter and Forscher (2001)(this issue) begins to address how one such pathway, an Src family tyrosine ki...

in this study, the mf285 tractor engine vibrations resulting from the use of different combinations of biodiesel, bioethanol and diesel as fuel were studied. engine vibrations in three directions and three engine speeds: 1000, 1600 rpm 2000 and ten fuel level was measured. to investigate the influence of measured parameters on engine vibration of factorial design in a randomized complete block ...

Compressors are a primary cause for numerous HV AC noise and vibration problems. Typically, vibration energy is transmitted from the compressor to the chassis through piping and isolation mounts. The design of these attachments is thus of paramount importance in determining the dynamic performance of a HV AC unit. Below certain frequencies, compressor vibration can be well described as a rigid-...

Salmonella carriers (16 adults) All had received previous antibiotics without effect. None were given during the trial. Criterion of "cure": Three negative daily post-treatment swabs. Dosage: Two tablets six hourly for seven days. Cures: Three only. Use in thrush (Candida albicans) post-antibiotic infection Perianal itching and soreness was rapidly cured in six adults, observed in general pract...

Using permanent magnets on a rotor can both simplify the design and increase the efficiency of electric machines compared to using electromagnets. A drawback, however, is the lack of existing automated assembly methods for large machines. This paper presents and motivates a method for robotized surface mounting of permanent magnets on electric machine rotors. The translator of the Uppsala Unive...

We present a micro-robotic system for protein crystal micro-manipulation. The specific task we focus on is the automated crystal mounting for X-ray data collection. The system features a set of newly designed micropositioner endeffectors we call micro-shovels which address some limitations of the traditional cryogenic loops. We have used micro-electrical mechanical system (MEMS) techniques to d...

in this paper, the amount of wasted heat from different parts of a 12 liter compression ignition engine and their potential for waste heat recovery were investigated. then, two-stage configuration of organic rankine cycle was introduced for simultaneous heat recovery from exhaust gases and coolant. finally, parameters like hybrid generated power, engine thermal efficiency and brake specific fue...

Automatic detection of cow’s estrus is important in cattle management. This paper proposes a method of estrus detection by checking cattle mounting activity automatically. We use a video sensor and apply computer vision techniques to detect the mounting activity. Especially, we derive motion vectors in order to distinguish the mounting activity from other activities, based on computer vision te...