........................................................................................................... 6 ORIGINAL ARTICLES ......................................................................................... 8 ABBREVIATIONS ................................................................................................. 9 INTRODUCTION .....................................................

The transfer of preimplantation embryos to a surrogate female is a required step for the production of genetically modified mice or to study the effects of epigenetic alterations originated during preimplantation development on subsequent fetal development and adult health. The use of an effective and consistent embryo transfer technique is crucial to enhance the generation of genetically modif...

The quality of oocytes-embryos can be determined by several techniques, including morphological, molecular, cellular and biochemical ones. The morphological methods of female gamete or embryo quality assessment often use thе following in vitro manipulation procedures such as: in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro embryo production (IVP). However, these methods are...

The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in vitro embryo production). Moreover, superstimulation entails prior administration of hormones tha...

Numerous reports on reproductive pathology in all rhinoceros species illustrate the abundance of female infertility in captive populations. In infertile rhinoceroses, oocyte collection and embryo production could represent the best remaining option for these animals to reproduce and to contribute to the genetic pool. We report here on superstimulation, repeated oocyte recovery, and attempted in...

Hamidu, J.A., J.J.R. Feddes, C.A. Ouellette and G.M. Fasenko. 2010. Measurement of avian embryo respiration by an indirect calorimetric system. Canadian Biosystems Engineering/Le génie des biosystèmes au Canada. 52: 4.9 4.14. Accurate measurement of embryonic respiration can be used to determine the viability of the chicken embryo and the chick upon hatching. This measurement is based on both t...

Increasing infertility is one of the most serious health problems today. Over past few years, we have had opportunity to follow progress technologies focused on production embryos in vitro (i.e., fertilization and intracytoplasmic sperm injection, genetic engineering, or somatic cell nuclear transfer. Oocyte maturation important processes vitro. Despite recent this field, developmental competen...

The Zika virus (ZIKV) epidemic brought new discoveries regarding arboviruses, especially flaviviruses, as ZIKV was described sexually and vertically transmitted. latter shows severe consequences for the embryo/fetus, such congenital microcephaly deficiency of neural system, currently known Congenital Syndrome (CZS). To better understand dynamics in trophoblastic cells present first trimester pr...