Hybridomas producing monoclonal antibodies (MAbs) directed against P. vivax erythrocytic stage antigens were isolated. Here, we describe the production of six MAbs. Culture supernatant from growing hybridomas was tested for their reactivity with P. vivax erythrocytic stages by ELISA against P. vivax crude (PvC) antigen and by immunofluorescence assay on P. vivax infected erythrocyte smears. P. ...

cucumber mosaic virus (cmv) is one of the causal agents of viral diseases that infected mint (mentha spp.). cmv is a member of the cucumovirus genus in  bromoviridae family. the virus is naturally transmitted by aphids in a non-persistent manner.during  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in tehran provi...

The vgf gene has been shown to be involved in several metabolic pathways. Because the pancreas is crucial to metabolism and food intake, we studied the VGF peptides in bovine, rat, and pig Langherans islets using antisera raised against specific sites along the primary sequence of the rat/mouse and human VGF protein precursor. Whereas almost all of the pancreatic endocrine cells expressed vgf m...

AIM To evaluate the efficacy of recombinant outer membrane proteinC (rOmpC) based enzyme-linked immunoassay (ELISA) for the diagnosis of salmonellosis in poultry. MATERIALS AND METHODS Three antigens were prepared, and the indirect ELISA was standardized using the antigens and the antiserum raised in chicken against Omp and rOmpC. Sera were collected from a total of 255 apparently healthy fie...

BACKGROUND Rhizomania is one of the most devastating diseases of sugar beet. It is caused by Beet necrotic yellow vein virus (BNYVV) transmitted by the obligate root-infecting parasite Polymyxa betae. Beta macrocarpa, a wild beet species widely used as a systemic host in the laboratory, can be rub-inoculated with BNYVV to avoid variation associated with the presence of the vector P. betae. To b...

The serological cross-reactivity between lipopolysaccharides (LPS) of S. fidelis KMM3582(T) and rabbit anti-O P. mirabilis antibodies was tested. Using ELISA and Western blot cross-reactivity between S. fidelis LPS and antisera against P. mirabilis O14, O3 LPSs was found. The observed cross-reaction may suggest that anti-P. mirabilis S1959 (O3) antibodies may bind to the internal part of S. fid...

Cucumber mosaic virus (CMV) is one of the causal agents of viral diseases that infected mint (Mentha spp.). CMV is a member of the cucumovirus genus in  Bromoviridae family. The virus is naturally transmitted by aphids in a non-persistent manner.During  growing season 2007-2008, leaf samples from plants of mint fields with symptoms of viral disease were collected in mint fieldas in Tehran provi...

BACKGROUND Pigeon breeder's disease is an extrinsic allergic alveolitis in the lungs of sensitised people, caused by hypersensitivity reactions to inhaled pigeon antigens. Antigens from different sources of the animal are used for diagnostic purposes, with serum being the most widely used. Bloom is rarely used; very little is known of its antigenicity and diagnostic performance, particularly wh...

Choline acetyltransferase (CATase; acetyl-CoA:choline O-acetyltransferase, EC 2.3.1.6) has been purified from bovine caudate nucleus. The specific activity of the pure enzyme was 120-160 mumol of acetylcholine formed per mg per min. The purified enzyme separated into two bands (band A and band B) when electrophoresed in a pH 4.3 gradient gel. Both bands exhibited CATase activity. Antisera were ...