Bone-marrow stromal cells can differentiate into multiple mesenchymal lineages including cartilage and bone. When these cells are seeded in high-density 'pellet culture', they undergo chondrogenesis and form a tissue that is morphologically and biochemically defined as cartilage. Here, we show that dual chondro-osteogenic differentiation can be obtained in the same micromass culture of human bo...

This article describes two kinds of "Cell Programmable Assay" (CPA) chips that utilize passive pumping for the culture and autonomous staining of cells to simply common protocols. One is a single timer channel CPA (sCPA) chip that has one timer channel and one main channel containing a cell culture chamber. The sCPA is used to culture and stain cells using Hoechst nuclear staining dye (a 2 step...

In vitro culture of single cells facilitates biological studies by deconvoluting complications from cell population heterogeneity. However, there is still a lack of simple yet high-throughput methods to perform single cell culture experiments. In this paper, we report the development and application of a microfluidic device with a dual-well (DW) design concept for high-yield single-cell loading...

Recent years have seen a transformation in thinking about the nature of culture. Rather than viewing culture in opposition to biology, a growing number of researchers now regard culture as subject to evolutionary processes. Recent developments in this field have shifted some of the traditional academic fault lines. Alliances are forming between researchers trained in anthropology, evolutionary ...

Bilineage differentiation along both the T lymphoid and the myeloid lineage while in in vivo diffusion chamber (DC) and in vitro suspension culture was observed in a case of acute unclassified leukemia (null-AL) and t(4;17). Prior to culture, the blast cells were TdT and la positive but did not express any lineage-specific antigenic markers. Furthermore, the immunoglobulin heavy chain and T cel...

This article examines The Beatles’ classic animated film, Yellow Submarine (dir. George Dunning, 1968) in terms of its appeal to multi-demographic audiences. While it has become de rigueur argue favour as an artefact the late-1960s counter-culture, status a children’s film largely been overlooked. will that invites, and is able sustain, range interpretations, particularly regarding dual quintes...

dual job holding is widespread in iran’s labor market, 18% of work force are holding second job in 1993, which increased to 19% in 2003. using a rich three years panel data-set of socioeconomic characteristics of iranian households, the article aims to examine the determinants of dual job holding across the individuals who have participated in the labor market. since the issue of dual job holdi...