The study reported herein utilized the Agrobacterium tumefaciens-induced potato disc tumor assay. The objective was to verify the detection of antineoplastic activity in the potato disc tumor induction assay, regardless of the mode of antineoplastic drug action. Camptothecin, paclitaxel, podophyllin, vinblastine and vincristine were tested, each with a different mode of action. All drugs tested...

Objective: To determine the phytochemical constituents, antioxidant and anticancer activities of Macranga peltata leaf extracts on A549 P 51 human lung cancer cell lines.
 Methods: Leaf sample was subjected to soxhlet extraction method screening done using different biochemical tests. The activity tested 2,2–diphenyl-1-picrylhydrazyl, ferric ion reducing power assay 2,2–azino bis 3–ethyl b...

The aim of the present study was to evaluate cytotoxic potential a new complex silver(I) with furosemide (Ag-FSE) over panel tumor and non-tumor human cells, as well its genotoxicological safety. According results obtained, cell line derived from colorectal adenocarcinoma (Caco-2) most sensitive Ag-FSE complex, among cells non-transforming fibroblasts known GM07492A, which stimulated characteri...

In vitro chemosensitivity of urological cancers was assessed by a human tumor colony forming assay (HTCA) and a 3H-thymidine incorporation assay. Primary tumor cells from 160 of 253 (63%) urological cancers showed adequate colony growth (greater than 30 colonies per well), and there was a 57% true positive and 100% true negative rate for predicting clinical response of anticancer agents. On the...

OBJECTIVE Lily of the valley is a poisonous plant due to the presence of the cardiac glycoside convallatoxin. We compared two immunoassays (LOCI digoxin assay and iDigoxin assay) for rapid detection of convallatoxin if present in human serum. MATERIALS AND METHODS Aliquots of a drug free serum pool and a digoxin serum pool were supplemented with microliter amounts of lily of the valley extrac...

Cassette dosing is a procedure for higher-throughput screening in drug discovery to rapidly assess pharmacokinetics of large numbers of candidate compounds. In this procedure, multiple compounds are administered simultaneously to a single animal. Blood samples are collected, and the plasma samples obtained are analyzed by means of an assay method such as liquid chromatography coupled to tandem ...

In the present study, a nanoporous metal organic framework (MOF) based on iron metal and amino terephthalate ligand MIL-101-NH2-Fe has been used as a carrier for loading and in vitro release of 5-flurouracil (5-FU) anticancer drug. The 5-FU drug loaded MOF was 13 wt % by using thermogravimetric analysis (TGA). The 5-FU release was monitored under physiological condition at 37°C, pH 7.4 in simul...